Method for high sensitivity detection of MYD88 mutations
View Patent ↗A method for increasing sensitivity for detecting minority mutations in MYD88 uses a locked nucleic acid oligo to block amplification of wild-type DNA in DNA isolated from patient FFPE tissue, bone marrow aspirate or peripheral blood samples during PCR while still allowing sequencing and visualization of the PCR product. Further improvement to the sensitivity may be achieved by using a uracil DNA-glycosylase treatment to remove sequence artifacts commonly found in formalin-fixed, paraffin-embedded tissue.
1. A method for detecting MYD88 mutations in a patient sample, the method comprising:
isolating DNA from a sampling bone marrow aspirate (BM), fresh peripheral blood (PB), and formalin-fixed, paraffin-embedded (FFPE) tissues sample obtained from a patient suspected of having a condition associated with MYD88 mutations;
performing PCR on the extracted DNA to produce amplified DNA while blocking amplification of wild-type DNA with an LNA oligonucleotide comprising SEQ ID NO. 5;
sequencing the amplified DNA in an automated sequencer;
analyzing an output of the automated sequencer to identify MYD88 mutations in the sequence.
2. The method of claim 1 , further comprising, prior to sequencing, applying a uracil DNA-glycosylase treatment to the sample to remove sequence artifacts.
3. The method of claim 1 , wherein the amplification is performed with the following primers comprising SEQ ID NO. 1 and SEQ ID No. 2.
4. The method of claim 1 , wherein the step of sequencing is performed using a sequencing method selected from the group consisting of Sanger sequencing, next generation sequencing, polymerase chain reaction, pyrosequencing, dye sequencing, sequencing by synthesis, and ion semiconductor sequencing.
5. The method of claim 1 , wherein the MYD88 mutation is associated with a cancer is selected from the group consisting of Waldenström's Macroglobulinemia (WM), diffuse large B-Cell lymphoma (DLBCL), monoclonal gammopathy of unknown significance (MGUS), and splenic marginal zone lymphoma (SMZL).
6. A method for analyzing a patient sample for presence or absence of MYD88-associated cancer, the method comprising:
extracting DNA from one or more samples obtained from a patient suspected of having a condition associated with MYD88 mutations;
performing PCR on the extracted DNA to produce amplified DNA while blocking amplification of wild-type DNA using an LNA oligonucleotide with SEQ ID NO. 5, wherein amplification is performed with primers comprising SEQ ID NO. 1 and SEQ ID NO. 2;
sequencing the amplified DNA in an automated sequencer; and
analyzing an output of the automated sequencer to identify MYD88 mutations in the sequence.
7. The method of claim 6 , wherein the one or more samples comprise bone marrow aspirate (BM), fresh peripheral blood (PB), and formalin-fixed, paraffin-embedded (FFPE) tissues.
8. The method of claim 6 , further comprising, prior to sequencing, applying a uracil DNA-glycosylase treatment to the one or more samples to remove sequence artifacts.
9. The method of claim 6 , wherein the MYD88-associated cancer is selected from the group consisting of Waldenström's Macroglobulinemia (WM), diffuse large B-Cell lymphoma (DLBCL), monoclonal gammopathy of unknown significance (MGUS), and splenic marginal zone lymphoma (SMZL).