Methods of using regenerative cells to promote epithelialization or neodermis formation
Cells present in adipose tissue are used to promote wound healing in a patient. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of regenerative cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the regenerative cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, cells present in adipose tissue are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic benefit.
1. A method of inducing epithelialization or neodermis formation at a wound of the skin of a subject, comprising:
identifying a subject with an wound; and
administering a therapeutically effective amount of a concentrated population of adipose-derived regenerative cells comprising adipose-derived stem cells and endothelial progenitor cells to said subject sufficient to accelerate the healing of said wound, wherein said concentrated population of adipose-derived regenerative cells is obtained by acquiring an adipose tissue sample, separating away adipocytes and connective tissue, and washing and concentrating said adipose-derived regenerative cells.
2. The method of claim 1 , wherein the concentrated population of adipose-derived regenerative cells has not been cultured.
3. The method of claim 1 , wherein the concentrated population of adipose-derived regenerative cells is obtained from adipose tissue of the subject to whom the cells are administered.
4. The method of claim 1 , wherein said administering step comprises applying the concentrated population of adipose-derived regenerative cells directly to the wound.
5. The method of claim 1 , wherein the wound is a diabetic ulcer.
6. The method of claim 1 , wherein the subject has peripheral vascular disease.
7. The method of claim 1 , wherein said administering step comprises subcutaneous delivery.
8. The method of claim 1 , wherein said administering step comprises dermal delivery.
9. The method of claim 1 , wherein the subject is obese.
10. The method of claim 1 , wherein said administration step comprises administering multiple doses of said concentrated population of adipose-derived regenerative cells.
11. The method of claim 1 , further comprising:
providing adipose tissue to a self-contained adipose tissue processing unit comprising:
a tissue collecting container configured to receive and fully or partially disaggregate adipose tissue, wherein said tissue collecting container is coupled to a cell collection container wherein said coupling defines a closed system in which said fully or partially disaggregated adipose tissue directed from tissue collecting container to cell collection container is not exposed to the external environment; and wherein said cell collection container, further comprises a cell concentrator that facilitates separation of the cells in a suspension; and
an outlet that is structured to permit said concentrated population of adipose-derived regenerative cells comprising adipose-derived stem and endothelial progenitor cells to be removed from said adipose tissue processing unit;
obtaining the concentrated population of adipose-derived regenerative cells from said self-contained adipose tissue processing unit.
12. The method of claim 1 , wherein the therapeutically effective amount of the concentrated population of adipose-derived regenerative cells comprises a minimum concentration of between 1×10 5 and 1×10 7 adipose-derived stem cells.
13. The method of claim 1 , wherein the concentrated population of adipose-derived regenerative cells further comprises an additive.
14. The method of claim 13 , wherein said additive is selected from the group consisting of a cell differentiation factor, a growth promoter, an immunosuppressive agent, a medical device, or a combination thereof.
15. The method of claim 13 , wherein said additive is selected from the group consisting of other cells, tissue, and tissue fragments.
16. The method of claim 13 , wherein said additive comprises an angiogenic factor, or an arteriogenic factor.
17. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is applied to a biocompatible matrix.
18. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is applied to a bandage.
19. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is not exposed to the external environment prior to the administration step.
20. The method of claim 1 , wherein the concentrated population of adipose-derived regenerative cells is cryopreserved.