Separating target analytes using alternating magnetic fields
View Patent ↗The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.
1. A method for detecting a target analyte in a sample, the method comprising:
contacting a sample with magnetic particles comprising binding moieties specific for a target analyte;
flowing the sample through a channel including second moieties specific to the target analyte attached to at least one surface of the channel while applying alternating magnetic fields to capture, on the at least one surface of the channel, magnetic particles that are bound to said target analyte;
rinsing said channel to remove unbound components of said sample;
eluting bound magnetic particle/second moiety/target analyte complexes from the at least one surface of the channel; and
detecting the eluted magnetic particle/second moiety/target analyte complexes, wherein the magnetic particle/second moiety/target analyte complexes are not examined in situ on the at least one surface of the channel.
2. The method of claim 1 , wherein detecting comprises the use of one or more processes selected from the group consisting of lysis, hybridization, cytometry, nucleic acid testing (NAT), polymerase chain reaction (PCR), and nuclear magnetic resonance (NMR).
3. The method of claim 1 , wherein the target analyte is a bacterium.
4. The method of claim 1 , wherein the sample is human tissue or body fluid.
5. The method of claim 4 , wherein the body fluid is blood.
6. The method of claim 1 , wherein the binding moieties are selected from the group consisting of antibodies, receptors, aptamers, proteins, and ligands.
7. The method of claim 1 , wherein the binding moieties are antibodies.