Platelet-derived growth factor compositions and methods of use thereof

1. An implant material comprising a porous calcium phosphate having incorporated therein a liquid comprising platelet derived growth factor (PDGF) at a concentration in a range of about 0.1 mg/mL to about 1.0 mg/mL in a buffer; wherein the calcium phosphate comprises interconnected pores, a porosity greater than 40%, and particles in a range of about 100 microns to about 5000 microns in size; and wherein the implant material does not comprise demineralized freeze-dried bone allograft.

2. The implant material of claim 1 , wherein the PDGF comprises recombinant PDGF.

3. The implant material of claim 1 , wherein the PDGF comprises recombinant human PDGF-BB.

4. The implant material of claim 1 , wherein the liquid comprises PDGF at a concentration of about 0.3 mg/mL in a buffer.

5. The implant material of claim 1 , wherein the liquid comprises PDGF at a concentration in a range of about 0.25 mg/mL to about 0.5 mg/mL in a buffer.

6. The implant material of claim 1 , wherein the calcium phosphate comprises tricalcium phosphate.

7. The implant material of claim 1 , wherein the calcium phosphate comprises particles in a range of about 100 microns to about 3000 microns in size.

8. The implant material of claim 1 , wherein the calcium phosphate comprises particles in a range of about 250 microns to about 1000 microns in size.

9. The implant material of claim 1 , wherein the implant material is resorbable such that at least 80% of the calcium phosphate is resorbed within one year of being implanted.

10. The implant material of claim 1 , wherein the incorporated liquid is adsorbed to or absorbed by the calcium phosphate.

11. The implant material according to claim 1 , further comprising collagen.

12. The implant material of claim 11 , wherein the PDGF comprises recombinant human PDGF-BB and the calcium phosphate comprises tricalcium phosphate.

13. The implant material of claim 11 , wherein the liquid comprises PDGF at a concentration of about 0.3 mg/mL in a buffer.

14. The implant material of claim 11 , wherein the calcium phosphate comprises particles in a range of about 100 microns to about 3000 microns in size.

15. The implant material of claim 11 , wherein the calcium phosphate comprises particles in a range of about 250 microns to about 1000 microns in size.

16. The implant material of claim 11 , wherein the implant material is resorbable such that at least 80% of the calcium phosphate is resorbed within one year of being implanted.

17. An implant material comprising a calcium phosphate having incorporated therein a liquid comprising platelet derived growth factor (PDGF) at a concentration in a range of about 0.1 mg/mL to about 1.0 mg/mL in a buffer; wherein the implant material comprises a porosity that facilitates cell migration into the implant material; wherein the calcium phosphate comprises interconnected pores and particles in a range of about 100 microns to about 5000 microns in size; and wherein the implant material does not comprise demineralized freeze-dried bone allograft.

18. The implant material of claim 17 , wherein said porosity is macroporosity.

19. The implant material of claim 17 , wherein said porosity comprises porous calcium phosphate particles having a porosity greater than 40%.

20. The implant material of claim 17 , wherein the PDGF comprises recombinant human PDGF-BB.

21. The implant material of claim 17 , wherein the liquid comprises PDGF at a concentration of about 0.3 mg/mL in a buffer.

22. The implant material of claim 17 , wherein the liquid comprises PDGF at a concentration in a range of about 0.25 mg/mL to about 0.5 mg/mL in a buffer.

23. The implant material of claim 17 , wherein the liquid comprises PDGF at a concentration in a range of about 0.2 mg/mL to about 0.75 mg/mL in a buffer.

24. The implant material of claim 17 , wherein the calcium phosphate comprises tricalcium phosphate.

25. The implant material of claim 17 , wherein the calcium phosphate comprises particles in a range of about 100 microns to about 3000 microns in size.

26. The implant material of claim 17 , wherein the calcium phosphate comprises particles in a range of about 250 microns to about 2000 microns in size.

27. The implant material of claim 17 , wherein the calcium phosphate comprises particles in a range of about 250 microns to about 1000 microns in size.

28. The implant material of claim 17 , wherein the implant material is resorbable such that at least 80% of the calcium phosphate is resorbed within one year of being implanted.

29. The implant material of claim 17 , further comprising collagen.

30. The implant material of claim 29 , wherein said porosity is macroporosity.

31. The implant material of claim 29 , wherein said porosity comprises porous calcium phosphate particles having a porosity greater than 40%.

32. The implant material of claim 29 , wherein the PDGF comprises recombinant human PDGF-BB and the calcium phosphate comprises tricalcium phosphate.

33. The implant material of claim 29 , wherein the liquid comprises PDGF at a concentration of about 0.3 mg/mL in a buffer.

34. The implant material of claim 29 , wherein the calcium phosphate comprises particles in a range of about 100 microns to about 3000 microns in size.

35. The implant material of claim 29 , wherein the calcium phosphate comprises particles in a range of about 250 microns to about 1000 microns in size.

36. An implant material comprising a calcium phosphate having incorporated therein a liquid comprising platelet derived growth factor (PDGF) at a concentration in a range of about 0.1 mg/mL to about 1.0 mg/mL in a buffer; wherein the calcium phosphate comprises interconnected pores, comprises particles in a range of about 100 microns to about 5000 microns in size, and is capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 25% of the calcium phosphate's own weight; and wherein the implant material does not comprise demineralized freeze-dried bone allograft.

37. The implant material of claim 36 , wherein the calcium phosphate is capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 50% of the calcium phosphate's own weight.

38. The implant material of claim 37 , wherein the calcium phosphate is capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 200% of the calcium phosphate's own weight.

39. The implant material of claim 38 , wherein the calcium phosphate is capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 300% of the calcium phosphate's own weight.

40. The implant material of claim 36 , further comprising collagen.

41. The implant material of claim 40 , wherein the calcium phosphate and collagen are capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 50% of the weight of the calcium phosphate and collagen.

42. The implant material of claim 40 , wherein the calcium phosphate and collagen are capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 200% of the weight of the calcium phosphate and collagen.

43. The implant material of claim 40 , wherein the calcium phosphate and collagen are capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 300% of the weight of the calcium phosphate and collagen.

44. An implant material comprising a calcium phosphate having incorporated therein a liquid comprising platelet derived growth factor (PDGF) at a concentration of about 0.3 mg/mL in a buffer; wherein the calcium phosphate comprises interconnected pores, comprises particles in a range of about 100 microns to about 5000 microns in size, and is capable of absorbing an amount of the liquid comprising PDGF that is equal to at least about 25% of the calcium phosphate's own weight; and wherein the implant material does not comprise demineralized freeze-dried bone allograft.

45. The implant material of claim 44 , further comprising collagen.