Plant promotor and 3′ UTR for transgene expression
This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a promoter and/or a 3′UTR from Zea mays Zrp2 gene. Some embodiments relate to a promoter from a Zea mays Zrp2 gene that is operably linked to a Zea mays Ubiquitin 1 intron and functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays Zrp2 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a 3′ UTR operably linked to:
a) a heterologous polylinker sequence; or
b) a non- Zea mays Zrp2 polynucleotide sequence,
wherein said 3′ UTR comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:3.
2. The nucleic acid vector of claim 1 , wherein said 3′ UTR is 500 bp in length.
3. The nucleic acid vector of claim 1 , wherein said 3′ UTR consists of a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:3.
4. The nucleic acid vector of claim 1 , further comprising a sequence encoding a selectable maker.
5. The nucleic acid vector of claim 1 , wherein said non- Zea mays Zrp2 polynucleotide sequence is a transgene.
6. The nucleic acid vector of claim 5 , wherein the transgene encodes a selectable marker or a gene product conferring insecticidal resistance, herbicide tolerance, expression of an RNAi, nitrogen use efficiency, water use efficiency, or nutritional quality.
7. The nucleic acid vector of claim 1 , further comprising a promoter polynucleotide sequence of SEQ ID NO:1, or a promoter polynucleotide sequence of SEQ ID NO:6, wherein the promoter sequence is operably linked to said polylinker or said non- Zea mays Zrp2 polynucleotide sequence.
8. The nucleic acid vector of claim 1 , further comprising an intron sequence.
9. The nucleic acid vector of claim 7 , wherein said promoter has below ground tissue specific expression.