Resistance alleles in soybean
View Patent ↗The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having iron deficiency chlorosis tolerance. A soybean plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a soybean plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.
1. A method of producing a soybean plant tolerant to iron deficiency chlorosis (IDC), comprising the steps of;
(a) isolating a nucleic acid from a soybean plant or part thereof;
(b) detecting in said nucleic acid, the presence of a plurality of single nucleotide polymorphism (SNP) markers associated with IDC tolerance in a soybean plant, wherein said markers are located within a chromosomal interval comprising physical positions 1035989-1401213 on Soybean chromosome 5, and further wherein said chromosomal interval comprises (i) a G allele at SY0152AQ; (ii) a G allele at SY0723BQ; (iii) a G allele at SY0724AQ; (iv) a SEQ ID NO: 301 insertion at SY1154AQ; and (v) an A allele at SY0153AQ as described in Table 2;
(c) identifying and/or selecting an iron deficiency chlorosis (IDC) tolerant soybean plant or part thereof;
(d) crossing the soybean plant of (c) with a second soybean plant not having said plurality of markers of step (b) in its genome;
(e) collecting seed from the cross in step (d); and
(f) growing a progeny soybean plant from said seed which comprises said plurality of markers in its genome, thereby producing a soybean plant with increased tolerance to IDC relative to a soybean plant not comprising said plurality of markers;
wherein said plurality of markers identifies a soybean plant comprising all of SNP alleles (i)-(v).
2. The method of claim 1 , wherein said chromosomal interval positions of (b) correspond to the reference genome of Williams 82.
3. The method of claim 1 , wherein the nucleic acid of (b) comprises SEQ ID NOs: 1-4 and 55.
4. The method of claim 1 , wherein a plant not tolerant to IDC is selected in the absence of any one of (i) a G allele at SY0152AQ; (ii) a G allele at SY0723BQ; (iii) a G allele at SY0724AQ; (iv) a SEQ ID NO: 301 insertion at SY 1154AQ; and (v) an allele at SY0153AQ as described in Table 2.
5. The method of claim 1 wherein IDC tolerance is exhibited by reduced yellow flash symptoms.
6. The method of claim 3 , wherein the iron deficiency chlorosis (IDC) tolerant soybean plant or part thereof is identified through use of any one of nucleotide probes comprising a nucleotide sequence as depicted in any one of SEQ ID NOs: 19-22; 37-40; 137 and 219.
7. The method of claim 3 , wherein the iron deficiency chlorosis (IDC) tolerant soybean or part thereof is identified through use of a PCR primer pair that anneals to any one of SEQ ID NOs: 1-4 or 55, wherein the primer pair is capable of initiating DNA polymerization by a DNA polymerase on a Glycine max nucleic acid template to generate a Glycine max amplicon.
8. The method of claim 7 , wherein the amplicon comprises a nucleotide sequence that is distinguishing for the presence or absence of alleles selected from the group consisting of: (i) a G allele at SY0152AQ; (ii) a G allele at SY0723BQ; (iii) a G allele at SY0724AQ; (iv) a SEQ ID NO: 301 insertion at SY 1154AQ; (v) an A allele at SY0153AQ and any combination of (i) through (v).