Microorganisms engineered for increased productivity
The application provides recombinant microorganisms with increased productivity with respect to control or wildtype microorganisms. The recombinant microorganisms can include a non-native gene encoding a SKP1 polypeptide or a CHORD-derived polypeptide. Increased productivity can be increased biomass or lipid productivity. These recombinant microorganisms can be used to produce products of interest.
1. A recombinant algal microorganism comprising a non-native nucleic acid molecule that comprises a nucleic acid sequence encoding an algal SKP1 polypeptide, wherein the recombinant algal microorganism has increased biomass or lipid productivity as compared to a control algal microorganism of the same species that does not comprise the non-native nucleic acid molecule, wherein the algal SKP1 polypeptide includes at least one tetramerization domain of Pfam PF03931 and at least one dimerization domain of Pfam PF01466, and wherein the algal SKP1 polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 28.
2. A recombinant algal microorganism according to claim 1 , wherein the non-native nucleic acid molecule further comprises a heterologous promoter operably linked to the nucleic acid sequence encoding said algal SKP1 polypeptide.
3. A recombinant algal microorganism according to claim 1 , wherein the algal SKP1 polypeptide has the amino acid sequence as set forth in SEQ ID NO: 28.
4. A recombinant algal microorganism according to claim 1 , wherein the recombinant algal microorganism is from the genus Nannochloropsis.
5. A method for producing a bioproduct, comprising culturing a recombinant algal microorganism according to claim 1 , producing a bioproduct therefrom, and recovering the bioproduct from the culture.
6. A method according to claim 5 , wherein the bioproduct is a biomass, a lipid, a polyketide, a terpenoid, a pigment, an antioxidant, a vitamin, a nucleotide, a nucleic acid, an amino acid, a carbohydrate, an alcohol, a hormone, a cytokine, a peptide, one or more proteins, or one or more polymers.
7. The method of claim 5 , wherein said recombinant algal microorganism is from the genus Nannochloropsis.
8. The method of claim 5 , wherein said culturing is under photoautotrophic conditions.