Clearing agent and mounting medium for microscopy
A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and/or a stain. The solution can also comprise derivatives and/or analogs of 2,2,2-trichloroethanol and/or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting. The solution can be used with specimens or tissues/cells/parts originating from animals, poultry, livestock, humans, higher plants, yeasts, molds, microorganisms, insects, mites, or reptiles.
1. A clearing agent and mounting solution, comprising:
(a) 10% to 97% (v/v) of trichloroethanol or derivative thereof;
(b) 0% to 5% (v/v) trichloroacetic acid;
(c) 12.5% to 50% (v/v) ethylene glycol, propylene glycol, polyethylene glycol, a polyethylene glycol derivative, or mixtures thereof, and/or derivatives thereof; and optionally water; wherein the refractive index of the solution is greater than or equal to 1.3810.
2. The clearing agent and mounting solution of claim 1 , further comprising a C1-C6 alcohol.
3. The clearing agent and mounting solution of claim 2 , wherein the C1-C6 alcohol comprises methanol, ethanol, or a mixture thereof.
4. The clearing agent and mounting solution of claim 1 , wherein the trichloroethanol derivative comprises mono- and poly-halogenated branched and unbranched alcohols, dials, glycol aldehydes, aldehyde-hydrates, hemi-acetals, acetals, ketals, aminals, hemi-aminals of at least 2 carbon units, and polymers thereof; wherein the branches comprise mono- or poly-halogenated aliphatic or aromatic groups containing hydroxyl, amino, ether, carboxyl, carboxyamido, carbonate, carbamyl, carbonyl-chloride, polyethylene-glycol, or aminoethanol groups.
5. The clearing agent and mounting solution of claim 1 , further comprising a solidification aid comprising polyethylene glycol, polyamide resin, polyvinylpyrrolidone, polyvinylalcohol, or mixtures thereof.
6. The clearing agent and mounting solution of claim 1 , further comprising glycerol.