Fusobacterium polypeptides and methods of use
The present invention provides isolated polypeptides isolatable from a Fusobacterium spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.
1. A composition comprising:
a polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl; and
an adjuvant.
2. The composition of claim 1 wherein the polypeptide has a molecular weight of 34 kDa.
3. The composition of claim 1 wherein the polypeptide has a molecular weight of 40 kDa.
4. The composition of claim 1 wherein the polypeptide has a molecular weight of 41 kDa.
5. The composition of claim 1 wherein the polypeptide has a molecular weight of 45 kDa.
6. The composition of claim 1 further comprising a second polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.
7. The composition of claim 1 further comprising a polypeptide having a molecular weight between 76 kDa and 86 kDa, between 62 kDa and 68 kDa, between 45 kDa and 53 kDa, between 34 kDa and 43 kDa, or between 24 kDa and 35 kDa and is natively expressed at a detectable level by a Fusobacterium spp. when incubated in medium comprising a metal chelator and is not natively expressed at a detectable level by the Fusobacterium spp. when the Fusobacterium spp. is grown in the medium without the metal chelator.
8. A composition comprising:
a polypeptide that specifically binds to an antibody that specifically binds to a polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl; and
an adjuvant.
9. The composition of claim 8 wherein the polypeptide has a molecular weight of 34 kDa.
10. The composition of claim 8 wherein the polypeptide has a molecular weight of 40 kDa.
11. The composition of claim 8 wherein the polypeptide has a molecular weight of 41 kDa.
12. The composition of claim 8 wherein the polypeptide has a molecular weight of 45 kDa.
13. The composition of claim 8 further comprising a second polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.
14. The composition of claim 8 further comprising a polypeptide having a molecular weight between 76 kDa and 86 kDa, between 62 kDa and 68 kDa, between 45 kDa and 53 kDa, between 34 kDa and 43 kDa, or between 24 kDa and 35 kDa and is natively expressed at a detectable level by a Fusobacterium spp. when incubated in medium comprising a metal chelator and is not natively expressed at a detectable level by the Fusobacterium spp. when the Fusobacterium spp. is grown in the medium without the metal chelator.
15. An immunizing composition comprising:
a polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl, the polypeptide provided in an amount effective to elicit an immune reaction in an animal when the composition is administered to the animal; and
an adjuvant.
16. The composition of claim 15 wherein the polypeptide has a molecular weight of 34 kDa.
17. The composition of claim 15 wherein the polypeptide has a molecular weight of 40 kDa.
18. The composition of claim 15 wherein the polypeptide has a molecular weight of 41 kDa.
19. The composition of claim 15 wherein the polypeptide has a molecular weight of 45 kDa.
20. The composition of claim 15 further comprising a second polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.
21. The composition of claim 15 further comprising a polypeptide having a molecular weight between 76 kDa and 86 kDa, between 62 kDa and 68 kDa, between 45 kDa and 53 kDa, between 34 kDa and 43 kDa, or between 24 kDa and 35 kDa and is natively expressed at a detectable level by a Fusobacterium spp. when incubated in medium comprising a metal chelator and is not natively expressed at a detectable level by the Fusobacterium spp. when the Fusobacterium spp. is grown in the medium without the metal chelator.
22. The composition of claim 15 wherein the animal is a mammal.
23. The composition of claim 22 wherein the mammal is an ungulate or a human.
24. The composition of claims 23 wherein the ungulate is bovine, ovine, or caprine.
25. The composition of claim 6 further comprising a third polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.
26. The composition of claim 13 , further comprising a third polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.
27. The composition of claim 20 , further comprising a third polypeptide having a molecular weight, as determined by sodium dodecyl sulfate gel electrophoresis, of from 34 kDa to 45 kDa and is expressed by a Fusobacterium spp. grown in medium with 2,2′-dipyridyl.