Cell culture support and associated method for cell growth and release
A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.
1. A cell culture support comprising:
a substrate;
a first coat immobilized on the substrate via non-covalent interaction, wherein the first coat comprises a homopolymer; and
a second coat immobilized on the first coat via non-covalent interaction, wherein the second coat comprises a dual stimuli responsive copolymer, and wherein the dual stimuli responsive copolymer is a block copolymer comprising poly(di(ethyleneglycol)methylether methacrylate)-co-polyacrylic acid.
2. The cell culture support of claim 1 , wherein the homopolymer is responsive to pH and/or ionic strength.
3. The cell culture support of claim 1 , wherein the homopolymer is selected from a group consisting of poly-L-lysine, poly-allylamine, poly-ethyleimine, and poly-vinylpyrrolidone.
4. The cell culture support of claim 1 , wherein the homopolymer comprises poly-L-lysine.
5. The cell culture support of claim 4 , further comprising a third coat immobilized on the second coat and a fourth coat immobilized on the third coat, wherein the third coat comprises a homopolymer and the fourth coat comprises a dual stimuli responsive copolymer.
6. The cell culture support of claim 5 , wherein the third coat comprises poly-L-lysine.
7. The cell culture support of claim 6 , wherein the fourth coat comprises poly(diethyleneglycolmethylether methacrylate)-co-polyacrylic acid.
8. The cell culture support of claim 7 , further comprising alternating coats of poly-L-lysine and poly(diethyleneglycolmethylether methacrylate)-copolyacrylic acid.
9. The cell culture support of claim 1 , wherein the block copolymer has a lower critical solution temperature in a range from about 10° C. to about 37° C.
10. The cell culture support of claim 9 , wherein the block copolymer is pH responsive in a pH range from about 3 to about 10.
11. The cell culture support of claim 1 , wherein the substrate comprises a material selected from a group consisting of glass, polymer, metal, ceramic, and a combination thereof.
12. The cell culture support of claim 1 , wherein the substrate comprises a microcarrier, a membrane, a fiber, a hollow fiber, a nonwoven fiber, a capillary, a vessel, a flask, a disc, a bead, a Petri dish, a plate, a fabric, a nano-fiber mat, a particle, a scaffold, or a foam.
13. The cell culture support of claim 12 , wherein the microcarrier is a bead.
14. A cell culture support comprising:
a substrate;
a first coat immobilized on the substrate via non-covalent interaction, wherein the first coat comprises a dual stimuli responsive copolymer, and wherein the dual stimuli responsive copolymer is a block copolymer comprising poly(di(ethyleneglycol)methylether methacrylate)-co-polyacrylic acid; and
a second coat immobilized on the first coat via non-covalent interaction, wherein the second coat comprises a homopolymer.
15. The cell culture support of claim 14 , wherein the homopolymer comprises poly-L-lysine.
16. The cell culture support of claim 15 , further comprising a third coat immobilized on the second coat and a fourth coat immobilized on the third coat, wherein the third coat comprises poly-(di(ethyleneglycol)methylether methacrylate)co- polyacrylic acid and the fourth coat comprises poly-L-lysine.
17. The cell culture support of claim 16 , further comprising alternating coats of poly(diethyleneglycolmethylether methacrylate)-co-polyacrylic acid and poly-L-lysine.
18. The cell culture support of claim 14 , wherein the substrate comprises a microcarrier, a membrane, a fiber, a hollow fiber, a capillary, a vessel, a flask, a disc, a bead, a Petri dish, a plate, a fabric, a nonwoven fiber, a nano-fiber mat, a particle, a scaffold, or a foam.
19. The cell culture support of claim 18 , wherein the microcarrier is a bead.