Cross-linked dehydrated placental tissue grafts and methods for making and using the same
Described herein are tissue grafts produced by contacting dehydrated placental tissue grafts with a cross-linking agent. The tissue grafts described herein provide barrier and prevent the migration of a bioactive agent from the wound. Thus, the tissue grafts enhance wound healing while preventing the undesirable migration of a bioactive agent from the wound. Methods for making and using the cross-linked grafts are also described herein.
1. A method for producing a dehydrated, cross-linked placental tissue graft, the method consisting of
i) contacting a dehydrated placental tissue graft with a solution comprising a cross-linking agent, thereby forming a cross-linked placental tissue graft; and
ii) dehydrating the cross-linked placental tissue graft formed in step 1), thereby forming the dehydrated, cross-linked placental tissue graft;
wherein the dehydrated placental tissue graft comprises at least two layers of amnion, or at least one layer of each of chorion and amnion; and
wherein at least one amnion layer comprises an amniotic fibroblast cell layer.
2. A method for enhancing wound healing in a subject comprising applying a tissue graft produced according to the method of claim 1 to the wound.
3. A method for reducing scar formation on or near the spine after a surgical procedure, the method comprising applying to a subject a tissue graft produced according to the method of claim 1 directly to the spinal dura of the subject, or a region near the spine.
4. The method of claim 3 , wherein the surgical procedure comprises a posterior procedure.
5. The method of claim 4 , wherein the posterior procedure is a laminectomy or discectomy.
6. The method of claim 3 , wherein the surgical procedure comprises an anterior procedure.
7. The method of claim 6 , wherein the anterior procedure is an Anterior Lumbar Interbody Fusion (ALIF) or Transforaminal Lumbar Interbody Fusion (TLIF).
8. A method for treating a dural tear in a subject, the method comprising applying directly to the dural tear a tissue graft produced according to the method of claim 1 .
9. The method of claim 2 , wherein the tissue graft comprises one or more additional placental components.
10. The method of claim 9 , wherein the one or more additional placental components comprise amnion, amnion membrane, chorion, Wharton's jelly, or any combination thereof.
11. The method of claim 2 , wherein the placental tissue graft comprises a laminate of amnion and chorion.
12. The method of claim 2 , wherein the placental tissue graft comprises a plurality of chorion and/or amnion membranes laminated to one another.
13. The method of claim 2 , wherein the dehydrated placental tissue graft was chemically dehydrated or thermally dehydrated.
14. The method of claim 2 , wherein the cross-linking agent comprises a sugar, a dialdehyde, an epoxide, a hydrazide, or a carbodiimide.
15. The method of claim 14 , wherein the sugar comprises D-ribose, glycerose, altrose, talose, ertheose, glucose, lyxose, mannose, xylose, gulose, arabinose, idose, allose, galactose, maltose, lactose, sucrose, cellibiose, gentibiose, melibiose, turanose, trehalose, isomaltose, or any combination thereof.
16. The method of claim 3 , wherein the tissue graft comprises one or more additional placental components.
17. The method of claim 16 , wherein the one or more additional placental components comprise amnion, amnion membrane, chorion, Wharton's jelly, or any combination thereof.
18. The method of claim 3 , wherein the placental tissue graft comprises a laminate of amnion and chorion.
19. The method of claim 3 , wherein the placental tissue graft comprises a plurality of chorion and/or amnion membranes laminated to one another.
20. The method of claim 3 , wherein the cross-linking agent comprises a sugar, a dialdehyde, an epoxide, a hydrazide, or a carbodiimide.
21. The method of claim 20 , wherein the sugar comprises D-ribose, glycerose, altrose, talose, ertheose, glucose, lyxose, mannose, xylose, gulose, arabinose, idose, allose, galactose, maltose, lactose, sucrose, cellibiose, gentibiose, melibiose, turanose, trehalose, isomaltose, or any combination thereof.
22. The method of claim 1 , wherein the placental tissue graft comprises at least one layer of each of chorion and amnion.
23. The method of claim 1 , wherein the dehydrated, cross-linked placental tissue graft is opaque.