Smear staining machine and smearing control method and device thereof
View Patent ↗A smear staining machine and a smearing control method and device thereof. The viscosity of the test sample is used for guiding the configuration of at least one smearing parameter. Because the viscosity of the test sample presents multiple influences of many effecting parameters, it is more suitable for presenting the characteristics of the test sample. Therefore, a better smearing effect could be acquired by referring the viscosity of the test sample to get the smearing parameter.
1. A smear control method for controlling a smear staining machine, comprising:
determining at least one smearing parameter according to a viscosity of a test sample comprises: measuring a viscosity characteristic of the test sample when the test sample flows through a pipeline under a preset condition, wherein the viscosity characteristic is a physical quantity influenced by the viscosity of the test sample when the test sample flows, and the physical quantity is a function of viscosity; and
controlling a smear action according to the smearing parameter;
wherein the viscosity characteristic of the test sample is a pressure comprising an absolute pressure or a pressure difference relative to an atmospheric pressure.
2. The smear control method of claim 1 , wherein the determining at least one smearing parameter further comprises:
processing the viscosity characteristic of the test sample to generate a processing result; and
determining the smearing parameter according to the processing result.
3. The smear control method of claim 2 , further comprising:
determining the smearing parameter according to a relative viscosity of the test sample, wherein the relative viscosity of the test sample is a viscosity of the test sample relative to a known apparent viscosity of a reference fluid at a smearing temperature, and wherein processing the viscosity characteristic of the test sample comprises:
comparing the viscosity characteristic of the test sample with a viscosity characteristic of the reference fluid when the reference fluid flows through the pipeline under the same preset condition to generate a comparison result; and
calculating the viscosity of the test sample according to the comparison result and the known apparent viscosity of the reference fluid and using the viscosity calculated as the relative viscosity of the test sample.
4. The smear control method of claim 3 , further comprising:
according to the relative viscosity, determining the smearing parameter using a table, a curve or a formula.
5. The smear control method of claim 3 , further comprising:
while measuring the viscosity characteristic of the reference fluid when the reference fluid flows through the pipeline under the same preset condition, measuring a first temperature in the pipeline; and wherein
the viscosity of the reference fluid is an apparent viscosity of the reference fluid at the first temperature.
6. The smear control method of claim 5 , further comprising:
while measuring the viscosity characteristic of the test sample when the test sample flows through the pipeline under the preset condition, measuring a second temperature in the pipeline.
7. The smear control method of claim 6 , wherein the viscosity characteristic of the test sample or the viscosity characteristic of the reference fluid is a pressure difference, and the reference fluid comprises a reference gas and a reference liquid when there is a liquid preserved in the pipeline, and the relative viscosity of the test sample is calculated through the below equation:
η
B
=
η
D_T
1
Δ
P
3
-
η
D_T
2
Δ
P
1
Δ
P
2
-
Δ
P
1
where η B is the relative viscosity of the test sample, η D . . . T1 is a viscosity of the reference liquid at the first temperature, η . . . T2 is a viscosity of the reference liquid at the second temperature, ΔP 1 is a pressure difference of the reference gas, ΔP 2 is a pressure difference of the reference liquid, and ΔP 3 is a pressure difference of the test sample.
8. The smear control method of claim 5 , wherein the viscosity characteristic of the test sample or the viscosity characteristic of the reference fluid is a pressure difference, and the reference fluid comprises a reference gas and a reference liquid when there is a liquid preserved in the pipeline, and the relative viscosity of the test sample is calculated through the below equation:
η
B
=
Δ
P
3
-
Δ
P
1
Δ
P
2
-
Δ
P
1
·
η
D
where η B is the relative viscosity of the test sample, η D is a viscosity of the reference liquid at the first temperature, ΔP 1 is a pressure difference of the reference gas, ΔP 2 is a pressure difference of the reference liquid, and ΔP 3 is a pressure difference of the test sample.
9. The smear control method of claim 3 , wherein the pipeline is a sampling pipeline of a smear staining machine, and where the method further comprises:
controlling the sampling pipeline to draw the reference fluid and the test sample with a same volume such that the reference fluid and the test sample flow through the pipeline in a same amount.
10. The smear control method of claim 3 , wherein the viscosity characteristic of the test sample or the viscosity characteristic of the reference fluid is a velocity.
11. The smear control method of claim 2 , further comprising:
determining the smearing parameter according to a viscosity ratio between the test sample and a reference fluid; and wherein processing the viscosity characteristic of the test sample comprises:
comparing the viscosity characteristic of the test sample with a viscosity characteristic of the reference fluid when the reference fluid flows through the pipeline under the same preset condition to obtain the viscosity ratio between the test sample and the reference fluid.
12. The smear control method of claim 11 , further comprising:
while measuring the viscosity characteristic of the test sample when the test sample flows through the pipeline under the preset condition, measuring an ambient temperature; and
according to the viscosity ratio and the ambient temperature, determining the smearing parameter using a table, a curve or a formula.
13. The smear control method of claim 1 , wherein the reference fluid comprises a reference gas and a reference liquid when there is a liquid preserved in the pipeline, and the pressure of the reference fluid comprises a pressure of the reference gas and a pressure of the reference liquid.
14. The smear control method of claim 13 , wherein the reference liquid is an isosmotic solution for the test sample.