METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS
Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
1 . A method of introducing a molecule of interest into a plant cell having a cell wall, the method comprising:
providing the plant cell having a cell wall;
coating a nanoparticle with a molecule of interest, wherein the nanoparticle is a gold nanoparticle or a quantum dot, and wherein the molecule of interest is an herbicide or a fungicide;
placing the cell having a cell wall and the coated nanoparticle in contact with each other; and
allowing uptake of the nanoparticle and the molecule of interest into the cell comprising a cell wall.
2 . The method according to claim 1 , wherein coating a nanoparticle with a molecule of interest comprises immobilizing the molecule of interest via noncovalent absorption on the surface of the nanoparticle.
3 . The method according to claim 1 , further comprising absorbing the molecule of interest into the nanoparticle.
4 . The method according to claim 1 , wherein the plant cell comprising a cell wall is selected from the group consisting of tobacco, carrot, maize, canola, rapeseed, cotton, palm, peanut, soybean, Oryza sp., Arabidopsis sp., Ricinus sp., and sugarcane cells.
5 . The method according to claim 1 , wherein the plant cell is from a tissue selected from the group consisting of embryo, meristematic, callus, pollen, leaves, anthers, roots, root tips, flowers, seeds, pods and stems.
6 . The method according to claim 1 , further comprising derivatizing the surface of the nanoparticle.
7 . The method according to claim 1 , further comprising selecting cells that have stably integrated the molecule of interest.
8 . The method according to claim 7 , wherein the selected cells are regenerable cells.
9 . The method according to claim 8 , further comprising regenerating a plant from the selected cells.
10 . The method according to claim 1 , further comprising coating the nanoparticle with a second molecule of interest.
11 . The method according to claim 1 , wherein the second molecule of interest is selected from the group consisting of amino acids, polypeptides, enzymes, peptide hormones, glycol-peptides, signaling peptides, and antibodies.
12 . The method according to claim 1 , wherein the second molecule of interest is selected from the group consisting of messengers, second messengers, and cAMP.
13 . The method according to claim 1 , wherein the second molecule of interest is selected from the group consisting of sugars, fats, vitamins, herbicides, fungicides, and antibiotics.
14 . A method of introducing a molecule of interest into a plant cell having a cell wall, the method comprising:
providing the plant cell having a cell wall;
coating a nanoparticle with a molecule of interest, wherein the nanoparticle is a gold nanoparticle or a quantum dot, and wherein the molecule of interest is selected from the group consisting of a sugar, a fat, a vitamin, a drug, and an antibiotic;
placing the cell having a cell wall and the coated nanoparticle in contact with each other; and
allowing uptake of the nanoparticle and the molecule of interest into the cell comprising a cell wall.
15 . The method according to claim 14 , wherein coating a nanoparticle with a molecule of interest comprises immobilizing the molecule of interest via noncovalent absorption on the surface of the nanoparticle.
16 . The method according to claim 14 , further comprising absorbing the molecule of interest into the nanoparticle.
17 . The method according to claim 14 , wherein the plant cell comprising a cell wall is selected from the group consisting of tobacco, carrot, maize, canola, rapeseed, cotton, palm, peanut, soybean, Oryza sp., Arabidopsis sp., Ricinus sp., and sugarcane cells.
18 . The method according to claim 14 , wherein the plant cell is from a tissue selected from the group consisting of embryo, meristematic, callus, pollen, leaves, anthers, roots, root tips, flowers, seeds, pods and stems.
19 . The method according to claim 14 , further comprising derivatizing the surface of the nanoparticle.
20 . The method according to claim 14 , further comprising selecting cells that have stably integrated the molecule of interest.
21 . The method according to claim 20 , wherein the selected cells are regenerable cells.
22 . The method according to claim 21 , further comprising regenerating a plant from the selected cells.
23 . The method according to claim 14 , further comprising coating the nanoparticle with a second molecule of interest.
24 . The method according to claim 14 , wherein the second molecule of interest is selected from the group consisting of amino acids, polypeptides, enzymes, peptide hormones, glycol-peptides, signaling peptides, and antibodies.
25 . The method according to claim 14 , wherein the second molecule of interest is selected from the group consisting of messengers, second messengers, and cAMP.
26 . The method according to claim 14 , wherein the second molecule of interest is selected from the group consisting of sugars, fats, vitamins, herbicides, fungicides, and antibiotics.