METHOD FOR PRODUCING USEFUL PROTEIN USING PLANT
Provided is a method for producing a protein with a plant, comprising a step of infecting the plant with Agrobacterium having a polynucleotide encoding a target protein, and a step of expressing the target protein in the plant by further cultivating the plant after infection, wherein production efficiency of the target protein is improved by imparting a damaging stimulus to the roots of the plant.
1 . A method for producing a protein with a plant, comprising:
cultivating a plant capable of being infected with Agrobacterium using a hydroponic cultivation method,
infecting the plant grown using a hydroponic cultivation method by contacting with an infecting solution containing Agrobacterium having a polynucleotide encoding a target protein, and
expressing the target protein in the plant by further cultivating the plant after infection; wherein,
a damaging stimulus is imparted to the roots of the plant.
2 . The method for producing a protein according to claim 1 , wherein the damaging stimulus comprises cutting at least a portion of the roots of the plant.
3 . The method for producing a protein according to claim 2 , wherein the portion of the roots that is cut is 0.01% by mass to 50% by mass of the total fresh weight of the plant roots.
4 . The method for producing a protein according to claim 2 , wherein cutting of the roots is carried out prior to infecting.
5 . The method for producing a protein according to claim 1 , further comprising purifying and/or recovering the target protein from the plant after expressing.
6 . The method for producing a protein according to claim 1 , wherein the target protein is a medicinal protein.
7 . The method for producing a protein according to claim 1 , wherein the plant is Nicotiana benthamiana.
8 . A method for producing a protein with a plant, comprising:
infecting the plant with Agrobacterium having a polynucleotide encoding a target protein, and
expressing the target protein in the plant by further cultivating the plant after infection; wherein,
a damaging stimulus is imparted to the roots of the plant.
9 . The method for producing a protein according to claim 8 , wherein the damaging stimulus is imparted to the roots of the plant prior to infection.
10 . The method for producing a protein according to claim 8 , wherein the damaging stimulus is imparted to the roots of the plant simultaneous to or after infection.