SYSTEMS AND METHODS FOR POINT-OF-CARE DETERMINATION OF LDL-C AND LDL-P

1 . A system for testing for LDL-C and LDL-P, the system comprising:

a first lateral flow test strip and a second lateral flow test strip;

a dosing area, the dosing area interconnected with the first and second lateral flow test strips;

a collector for collecting a sample; and

a first mixer for receiving the sample from the collector, the mixer including buffers for mixing with the sample, the first mixer for dosing the sample pad a first time, wherein the first lateral flow test strip provides for the detection of LDL-C and the second lateral flow test strip provides for the detection of LDL-P.

2 . The system of claim 1 , further comprising:

a second mixer, the second mixer containing an enzyme formulation, the enzyme formulation for dosing the sample pad.

3 . The system of claim 1 , wherein the first lateral flow test strip includes an antibody-antigen stripe and an antigen stripe.

4 . The system of claim 3 , wherein the antibody-antigen stripe is an anti Apo B Ab-latex conjugate.

5 . The system of claim 3 , wherein the antibody-antigen stripe includes blue particles connected to the anti Apo B-100 antibody.

6 . The system of claim 4 , wherein the antigen stripe is an Apo B-100 antigen stripe, located in a direction of flow distal from the dosing area on the first lateral flow test strip.

7 . The system of claim 6 , wherein the second lateral flow test strip includes an anti Apo B-100 antibody stripe.

8 . The system of claim 1 , wherein the first and second lateral flow test strips include conjugate, nitrocellulose, and nylon membranes.

9 . A method of testing for LDL-C and LDL-P, the method comprising:

providing a meter, a first lateral flow test strip, a second lateral flow test strip, a dosing area, the dosing area interconnected with the first and second lateral flow test strips, a collector for collecting a sample, and a first mixer;

collecting a sample with the collector;

mixing the sample with the mixer;

dosing the sample on the dosing area;

laterally flowing the sample across the first and second lateral flow test strips; and

reading the first lateral flow test strip to determine a concentration of LDL-P in the sample.

10 . The method of claim 9 , further comprising:

providing a sampler, the sampler containing an enzyme formula;

dosing the dosing area with the enzyme formula; and

reading the second lateral flow test strip with the meter to determine a concentration of LDL-C in the sample.

11 . The method of claim 10 , wherein the first lateral flow test strip includes an antibody-antigen stripe and an antigen stripe.

12 . The method of claim 11 , wherein the antibody-antigen stripe is an anti Apo B Ab-latex conjugate.

13 . The method of claim 11 , wherein the antibody-antigen stripe includes blue particles connected to the anti Apo B-100 antibody.

14 . The method of claim 12 , wherein the antigen stripe is an Apo B-100 antigen stripe, located in a direction of flow distal from the dosing area on the first lateral flow test strip.

15 . The method of claim 14 , wherein the second lateral flow test strip includes an anti Apo B-100 antibody stripe.

16 . The method of claim 15 , wherein the first and second lateral flow test strips include conjugate, nitrocellulose, and nylon membranes.

17 . The method of claim 15 , further comprising, after the dosing of the sample:

flowing the sample to the antibody-antigen stripe of the first lateral flow test strip;

binding the anti Apo B-100 antibody to the LDL-P in the sample;

flowing the sample to the antigen stripe of the first lateral flow test strip; and

capturing unbound portions of the anti Apo B-100 antibody to the antigen stripe.

18 . The method of claim 17 , further comprising, after the dosing of the sample:

flowing the sample to the anti Apo B-100 antibody stripe of the second lateral flow test strip; and

binding LDL to the anti Apo B-100 antibody stripe of the second lateral flow test strip.

19 . The method of claim 18 , further comprising, after the dosing with the enzyme formula:

flowing the enzyme formula to bound LDL, the bound LDL being a result of binding LDL to the anti Apo B-100 antibody stripe; and

reacting the enzyme formula with the bound LDL.

20 . The method of claim 19 , wherein the reading of the first lateral flow test strip and reading of the second lateral flow test strip include reading an optical property with the meter for each reading.