Nanoparticle compositions and methods of making and using the same
Water soluble graphene oxide nanoparticles (GO) are provided having graphene sheets containing carboxylic and hydroxyl groups. The diameter of the GO when present in a closed form ranges from about 40 nm to 120 nm. Eco-friendly methods are provided for producing the GO. Methods for reversible encapsulation of a molecule within water soluble carbon nanoparticles (wsCNP) including the GO nanoparticles are provided that allow for delivery of the wsCNP loaded with therapeutic and/or imaging agents to a subject in need by releasing the therapeutic/imaging agent upon an increase in pH above about 7.2. The wsCNP have been shown to cross the blood brain barrier in a mouse model of vascular dementia, and methods are provided for delivering the wsCNP loaded with a therapeutic and/or imaging molecule to the brain.
1. A method for making graphene oxide nanoparticles, the method comprising:
a) treating a material comprising one or a combination of wood charcoal, low grade coal, or carbonized plant biomass with a dilute alkali solution to form a mixture of components including insoluble material and a second solution;
b) separating the second solution from the insoluble material; and
c) neutralizing the second solution with an acid to form a precipitate upon standing; and
d) separating the precipitate from the neutralized second solution;
wherein the precipitate comprises graphene oxide nanoparticles having a plurality of sheets ranging in size from about 40 nm to 200 nm in the open form.
2. The method of claim 1 , wherein the dilute alkali solution is 10% sodium hydroxide.
3. The method of claim 1 , wherein the acid comprises hydrochloric acid.
4. The method of claim 1 , wherein the treating in dilute alkali solution is performed at a temperature ranging from about 25° C. to 40° C.
5. The method of claim 4 , wherein the temperature is about 40° C.
6. The method of claim 1 , wherein the treating with dilute alkali solution is performed until the solution turns a yellow-brown color.
7. The method of claim 1 , wherein the wood charcoal, low grade coal or carbonized biomass is in a powdered form.
8. The method of claim 1 wherein the wood charcoal, low grade coal or carbonized biomass is washed with an organic solvent prior to treatment with the dilute alkali solution to remove aromatic hydrocarbons and soluble organic compounds.
9. The method of claim 1 , further comprising washing the precipitate with cold water.