System and method for detecting target substances
View Patent ↗A method includes capturing a signal of a detection substrate exposed to a sample containing a target substance; determining that the detection substrate is in a testable state; and generating an assessment of the presence of the target substance in the sample.
1. A system for optical detection of target substances, comprising:
a test container comprising:
a sample processing chamber, and
a detection substrate fluidly connected to the sample processing chamber;
a housing defining a component volume and a test container lumen adjacent and optically connected to the component volume through a testing window, the test container lumen configured to removably receive the test container in an analysis configuration, wherein the detection substrate is adjacent the testing window in the analysis configuration;
an optical sensing subsystem mounted to the housing within the component volume, the optical sensing subsystem comprising:
an illuminator arranged proximal an end of the testing window and directed toward the testing window at an oblique angle, and
an imager arranged proximal a center of the testing window with an active face directed substantially toward the testing window, the imager arranged between the testing window and the illuminator; and
a processing module mounted to the housing and communicably connected to the optical sensing subsystem, the processing module configured to:
record an image of the detection substrate;
control the optical sensing subsystem, based on the drive element operation data and the image of the detection substrate;
determine a test output, based on the image of the detection substrate; and
provide the test output to a user.
2. The system of claim 1 , wherein in the analysis configuration:
a longitudinal axis of the test container is aligned at least partially with a gravity vector, and
a pressure force, at least partially generated by hydrostatic pressure of a processed sample, urges the processed sample contained by the sample processing chamber into fluid communication with the detection substrate.
3. The system of claim 2 , further comprising an orientation sensor, wherein the processing module is further configured to receive orientation data from the orientation sensor; wherein the optical sensing subsystem is controlled based on the orientation data, and the test output is determined based on the orientation data.
4. The system of claim 1 , wherein in the analysis configuration, the test container and the housing cooperatively isolate the detection substrate from ambient light.
5. The system of claim 1 , wherein the illuminator comprises a set of light emitters, wherein each of the set of light emitters is configured to emit light at a corresponding power level along a corresponding optical axis.
6. The system of claim 5 , wherein processing module is further configured to control the corresponding power level of each of the set of light emitters, comprising:
determining a distribution of radiant flux from the set of light emitters; and
controlling the light emitters to produce the determined radiant flux distribution at the surface of the detection substrate.
7. The system of claim 1 , wherein the imager comprises:
a detector configured to detect the image of the detection substrate; and
an imaging aperture configured to transform light scattered towards the imaging aperture from the surface of the detection substrate into the image of the detection substrate at the detector.
8. The system of claim 7 , wherein the processing module is configured to adjust an exposure time of the detector based on the recorded image of the detection substrate.
9. The system of claim 1 , wherein the processing module is configured to average pixel values of adjacent pixels of the detector and to record a spatially-averaged image of the detection substrate based on the averaged pixel values.