Polynucleotides encoding engineered imine reductases
View Patent ↗The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
1. An engineered polynucleotide encoding an engineered polypeptide having imine reductase activity, wherein said engineered polypeptide has at least 90% sequence identity to SEQ ID NO:2, wherein the amino acid residue at the position corresponding to position 198 of SEQ ID NO:2 has been replaced with an aliphatic, non-polar, polar, or acidic residue.
2. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide has at least 90% sequence identity to SEQ ID NO: 2 and comprises an alanine, histidine, proline, serine, or glutamic acid at the position corresponding to position 198 of SEQ ID NO: 2.
3. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide is capable of converting substrate compound (1a) pyruvate,
and substrate compound (2b) butylamine
to product compound (3b), N-2-(butylamino)propanoic acid,
under suitable reaction conditions.
4. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide is capable of converting substrate compound (1b) cyclohexanone,
and substrate compound (2a) L-norvaline
to product compound (3c), (S)-2-(cyclohexylamino)pentanoic acid,
under suitable reaction conditions.
5. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide is capable of converting substrate compound (1b) cyclohexanone,
and substrate compound (2b) butylamine
to product compound (3d), N-butylcyclohexanamine,
under suitable reaction conditions.
6. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide is capable of converting substrate compound (1i),
and substrate compound (2b)
to product compound (3n),
under suitable reaction conditions.
7. The engineered polynucleotide of claim 1 , wherein said engineered polypeptide is capable of converting substrate compound (1j),
and substrate compound (2b)
to product compound (3o),
under suitable reaction conditions.