Concentrated, inhalable ciprofloxacin formulation
A concentrated inhalable formulation of an antibiotic drug such as ciprofloxacin is disclosed. The antibiotic is formulated with sodium acetate and liposome which incorporate antibiotic. The formulation is aerosolized and inhaled for treatment of respiratory tract infections and other medical conditions.
1. A formulation, comprising:
free unencapsulated ciprofloxacin;
a pharmaceutically acceptable excipient; and
liposome-encapsulated ciprofloxacin wherein the liposomes are unilamellar, have an average particle size of 1 nanometer to 10 microns, and comprise cholesterol and hydrogenated soy phosphatidyl-choline (HSPC);
wherein the formulation is formulated for aerosolized delivery such that the liposomes maintain integrity when aerosolized, and provide a ciprofloxacin release rate of 0.5% to 20% per hour.
2. The formulation of claim 1 , wherein the liposomes have a diameter in a range from 20 nanometers to 1 micron.
3. The formulation of claim 2 , wherein the free ciprofloxacin comprises between about 1 and about 75% of the total free and liposome-encapsulated ciprofloxacin.
4. The formulation of claim 1 , wherein the liposomes provide a ciprofloxacin release rate of 2% to 10% per hour.
5. The formulation of claim 1 , further comprising:
an isotonic histidine buffer.
6. The formulation of claim 1 , wherein the formulation pH is 6.0.
7. The formulation of claim 1 , wherein the formulation osmolarity is 300 mOsm/kg.
8. The formulation of claim 1 , wherein the formulation is in an isotonic histidine buffer at pH 6.0, and an osmolarity of 300 mOsm/kg.
9. A formulation for aerosolized delivery, comprising:
free unencapsulated ciprofloxacin;
a pharmaceutically acceptable excipient; and
liposome-encapsulated ciprofloxacin wherein the liposomes comprise cholesterol and hydrogenated soy phosphatidyl-choline (HSPC), are unilamellar and wherein the liposomes are comprised of cholesterol and hydrogenated soy phosphatidyl-choline (HSPC), and 90% or more of the liposomes maintain integrity when aerosolized, and provide a ciprofloxacin release rate of 0.5% to 20% per hour.
10. The formulation of claim 9 , wherein the liposomes provide a ciprofloxacin release rate of 2% to 10% per hour.
11. The formulation of claim 9 , wherein the liposomes have a diameter in a range from 20 nanometers to 1 micron.
12. The formulation of claim 9 , wherein the free ciprofloxacin comprises between about 1 and about 75% of the total free and liposome-encapsulated ciprofloxacin.
13. The formulation of claim 9 , wherein the liposomes provide a ciprofloxacin release rate of 2% to 10% per hour.
14. The formulation of claim 9 , further comprising:
an isotonic histidine buffer.
15. The formulation of claim 9 , wherein the formulation pH is 6.0.
16. The formulation of claim 9 , wherein the formulation osmolarity is 300 mOsm/kg.
17. The formulation of claim 9 , wherein the formulation is in an isotonic histidine buffer at pH 6.0, and an osmolarity of 300 mOsm/kg.