IP Library › Granted Patent US 10,767,176
Granted Patent B2
US 10,767,176 · App. 15/299,549 · Granted Sep 8, 2020

CRISPR-based compositions and methods of use

Inventors: Michael Allen Collingwood (North Liberty, IA); Ashley Mae Jacobi (Tiffin, IA); Garrett Richard Rettig (Coralville, IA); Mollie Sue Schubert (Cedar Rapids, IA); Mark Aaron Behlke (Coralville, IA)
Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
C12N15/111C12N2310/10C12N2310/20C12N2310/315C12N2310/317C12N2310/321C12N2310/322C12N2310/3231C12N2310/346C12N2310/531C12N2320/51C12N2320/53
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 10,767,176
App. No.
15/299,549
Granted
Sep 8, 2020
Kind
B2
Abstract

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.

Claims (1)

1. An isolated tracrRNA selected from the group consisting of SEQ ID NOS:19-21, and 449-451, wherein the isolated tracrRNA is active in a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein endonuclease system.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 14, 2017
From: COLLINGWOOD, MICHAEL ALLEN; JACOBI, ASHLEY MAE; RETTIG, GARRETT RICHARD; SCHUBERT, MOLLIE SUE; BEHLKE, MARK AARON
To: INTEGRATED DNA TECHNOLOGIES, INC.
Reel/Frame 042014/0243 →