Methods for treating cancer using nucleic acids targeting MDM2 or MYCN
Aspects of the invention relate to methods for treating cancer by administering to a subject in need thereof a therapeutically effective amount of a nucleic acid molecule that is directed against a gene encoding mouse double minute 1 homolog (MDM1), mouse double minute 2 homolog (MDM2), mouse double minute 3 homolog (MDM3), mouse double minute 4 homolog (MDM4) or V-myc myelocytomatosis viral related oncogene (MYCN) for treating cancer. Further aspects of the invention relate to nucleic acid molecules and compositions comprising nucleic acid molecules.
1. A method for treating cancer, comprising administering to a subject in need thereof a therapeutically effective amount of a nucleic acid molecule that is directed against a gene encoding mouse double minute 2 homolog (MDM2),
wherein the nucleic acid molecule is an isolated double stranded nucleic acid molecule that comprises a sense strand and an antisense strand,
wherein the sense strand of the isolated double stranded nucleic acid molecule comprises SEQ ID NO: 727 (mC.mA.A.A.G.mA.A.G.mC.mU.A*mA*mA-Chl), and wherein the antisense strand of the isolated double stranded nucleic acid molecule comprises SEQ ID NO: 752 (P.mU.fU.fU.A.G.fC.fU.fU.fC.fU.fU.fU.G*fC*A*fC*A*fU*G).
2. The method of claim 1 , wherein the cancer is retinoblastoma, neuroblastoma, or glioblastoma.
3. The method of claim 1 , wherein the cancer is located in the eye.
4. The method of claim 3 , wherein the cancer is located in the retina.
5. The method of claim 1 , wherein the isolated double stranded nucleic acid molecule is in a composition formulated for topical delivery or is in a composition formulated for delivery to the eye.
6. The method of claim 5 , wherein the isolated double stranded nucleic acid molecule is in a composition formulated for intravitreal injection, subretinal injection, or subconjunctival administration.
7. The method of claim 1 , further comprising administering a second isolated double stranded nucleic acid molecule that is directed against a gene encoding a different protein, or that is directed against a gene encoding the same protein to the subject.
8. The method of claim 1 , wherein the isolated double stranded nucleic acid molecule is administered more than once.