IP Library › Granted Patent US 10,174,355
Granted Patent B2
US 10,174,355 · App. 15/307,582 · Granted Jan 8, 2019

Method for glycosylation of ginsenoside using a glycosyltransferase derived from panax ginseng

Inventors: Sun Chang Kim (Daejeon, KR); Gil Tsu Choi (Daejeon, KR); Suk Chae Jung (Daejeon, KR); Myung Keun Park (Daejeon, KR); Woo Hyun Kim (Daejeon, KR); Soo Hwan Lim (Daejeon, KR); Wan Taek Im (Daejeon, KR)
Assignees: Korea Advanced Institute of Science and Technology; Intelligent Synthetic Biology Center
C12P33/00C12N9/10C12N9/1051C12P33/20C12Y204/01017
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Quick Facts
Patent No.
US 10,174,355
App. No.
15/307,582
Granted
Jan 8, 2019
Kind
B2
Abstract

The present invention relates to a uridine diphosphate (UDP)-glycosyltransferase protein which has glycosylation activity for a hydroxyl group at the C-20 position of a protopanaxadiol (PPD)- or protopanaxatriol (PPT)-type ginsenoside, and a method for glycosylation of UDP using the same.

Claims (5)

1. A method for preparing a protopanaxadiol (PPD)- or protopanaxatriol (PPT)-type ginsenoside whose hydroxyl group at the C-20 position is glycosylated, comprising:

contacting a uridine diphosphate (UDP) glycosyltransferase protein with a PPD- or PPT-type ginsenoside having a hydroxyl group at the C-20 position to produce a glycosylated PPD- or PPT-type ginsenoside,

wherein the UDP glycosyltransferase protein is defined by the amino acid sequence of SEQ ID NO:1.

2. The method according to claim 1 , wherein the PPD- or PPT-type ginsenoside having the hydroxyl group at the C-20 position is one or more selected from the group consisting of PPD, Rh2, Rg3, and PPT.

3. The method according to claim 1 , wherein the method comprises one or more conversion step selected from the group consisting of a conversion of PPD to compound K (C—K), a conversion of Rh2 to F2, a conversion of Rg3 to Rd, and a conversion of PPT to F1.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 11, 2017
From: KIM, SUN-CHANG; CHOI, GIL TSU; JUNG, SUK CHAE; PARK, MYUNG KEUN; KIM, WOO HYUN; LIM, SOO HWAN; IM, WAN TAEK
To: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Reel/Frame 042670/0820 →
Priority Claims (1)
KR 10-2014-0052728 · Apr 30, 2014 · national
Continuity (1)
Related Publication 20170121750A1 · May 4, 2017