HAR-NDS-derived stem cells, method for separating same, and use thereof
The present invention relates to hyaluronic acid-rich node and duct system (HAR-NDS)-derived stem cells, a method for separating the same, and a use thereof and, more specifically, to node and ductal stem cells (NDSCs), which are adult stem cells having an ability to differentiate into HAR-NDS-derived neural cells, and hematopoietic stem cells having an ability to differentiate into blood cells. The present invention is capable of separating, from HAR-NDS, adult stem cells NDSCs and hematopoietic stem cells, which are not easy to obtain from bone marrow, peripheral blood and umbilical cord blood (cord blood), as an alternative source, and thus can be usefully used for treatment of brain diseases, neurological diseases, chronic infectious diseases, cancers, autoimmune diseases, organ regeneration treatments and various intractable diseases.
1. A method for obtaining hyaluronic acid-rich node and duct system (HAR-NDS)-derived node and ductal stem cells (NDSCs), comprising:
(a) staining, in vivo, a sample of the HAR-NDS of a mammal with one or more dyes selected from the group consisting of: alcian blue, methylene blue and Janus green B (JGB);
(b) obtaining the stained HAR-NDS sample from the mammal;
(c) isolating NDSCs within the HAR-NDS sample obtained in (b), wherein the isolated NDSCs express Sca-1 + , Lin − , and CD45 − , and wherein the isolated NDSCs express Oct4, Sox2, Nanog, and SSEA-1.
2. The method of claim 1 , wherein the HAR-NDS sample comprises a network structure comprising nodes and ducts on organ surfaces.
3. The method of claim 1 , wherein the HAR-NDS sample of step (b) is obtained by fluorescence-activated cell sorting.
4. The method of claim 1 , wherein step (a) comprises injecting the dye into a subject's lymphatic system.
5. The method of claim 1 , wherein the NDSCs are capable of differentiating into neuronal cells.
6. The method of claim 1 , wherein the HAR-NDS sample comprises a network structure comprising nodes and ducts in blood vessels.
7. The method of claim 1 , wherein the HAR-NDS sample comprises a network structure comprising nodes and ducts in lymphatics.
8. The method of claim 1 , wherein the HAR-NDS sample comprises a network structure comprising nodes and ducts in the skin.