Increase in meiotic recombination in plants by inhibiting an RECQ4 or TOP3A protein of the RTR complex
The invention relates to a method for increasing the frequency of meiotic recombination in plants, by inhibiting the RECQ4 or TOP3A protein, especially by mutagenesis or extinction of the RECQ4 or TOP3A gene coding for said protein. The invention can be used especially in the field of plant breeding and genetic mapping.
1. A process for increasing the frequency of meiotic crossovers in a plant said process comprising:
inhibiting in said plant, the expression or the function of:
a protein of the RTR complex known as RECQ4; or
both REQ4A and RECQ4B proteins if said plant belongs to the family of Brassicaceae and expresses two functional RECQ4 proteins, said RECQ4 protein having at least 40% sequence identity with the RECQ4 protein of SEQ ID NO: 1, and comprising a region having at least 60% sequence identity with the region which extends from positions 407 to 959 of the sequence SEQ ID NO:1; and
measuring the frequency of meiotic crossovers in said plant.
2. The process of claim 1 , further comprising, inhibiting in said plant, the expression or the function of at least one protein chosen from:
(i) a protein hereinafter referred to as FIDG, said protein having at least 45% sequence identity with the AtFIDG protein of SEQ ID NO:46 and containing an AAA-ATPase domain and a VSP4 domain, and
(ii) a protein hereinafter referred to as FANCM, said protein having at least 30% sequence identity with the AtFANCM protein of SEQ ID NO:45, and containing a DEXDc helicase domain and a HELICc helicase domain.
3. The process of claim 1 , wherein the inhibition of the RECQ4 is obtained by mutagenesis of the gene encoding said protein or of its promoter.
4. The process of claim 1 , wherein the inhibition of the RECQ4 is obtained by silencing the gene encoding said protein by expressing, in said plant, an interfering RNA targeting said gene.
5. The process of claim 1 , further comprising, inhibiting in said plant, the expression or the function of a protein known as TOP3A, said TOP3A protein having at least 50% sequence identity with the TOP3A protein of sequence SEQ ID NO: 2.
6. The process of claim 5 , wherein the inhibition of the TOP3A protein is obtained by mutagenesis of the gene encoding said protein or of its promoter.
7. The process of claim 6 , wherein the mutagenesis of the TOP3A protein comprises the introduction, into an allele of the TOP3A gene, of a mutation which results in the inactivation of at least one zinc finger domain of said TOP3A protein.
8. The process of claim 5 , the process further comprising the expression, in said plant, of a recombinant TOP3A protein comprising a TOPRIM domain and a Topoisomerase IA domain which have not been impaired and at least one zinc finger domain which is inactivated.
9. The process of claim 1 , wherein said plant is a monocotyledonous or dicotyledonous plant of agronomical interest comprising rapeseed, sunflower, potato, corn, wheat, barley, rye, sorghum, rice, soy, bean, carrot, tomato, zucchini, bell pepper, eggplant, turnip, onion, pea, cucumber, leek, artichoke, beetroot, cabbage, cauliflower, lettuces, endive, melon, watermelon, strawberry plant, apple tree, pear tree, plum tree, poplar tree, vine, cotton, rose, or tulip.