Enzymatic synthesis of soluble glucan fiber
View Patent ↗An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
1. A method to produce an alpha-glucan, said method comprising:
(a) combining at least sucrose and an isolated polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:10 under suitable aqueous reaction conditions to catalyze synthesis of water-soluble alpha-glucan comprising 100% alpha-1,6 glycosidic linkages, and
(b) optionally isolating the water-soluble alpha-glucan produced in step (a).
2. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 96% identity to SEQ ID NO:10.
3. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 97% identity to SEQ ID NO:10.
4. The method of claim 1 , wherein the method comprises step (b) of isolating the water-soluble alpha-glucan produced in step (a).
5. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 98% identity to SEQ ID NO:10.
6. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 99% identity to SEQ ID NO:10.