Stabilization of thrombocytes at ambient temperatures
Provided herein are formulations and methods for the stabilization of one or more thrombocytes at ambient temperatures. Also provided are formulations and methods for the stabilization of one or more thrombocytes in an inactivated state in a blood sample at ambient temperatures. Further provided are articles of manufacture and kits and methods for substantially stable storage of one or more thrombocyte at ambient temperatures.
1. A composition for substantially stable storage of one or more thrombocytes at ambient temperature, comprising a halogenated disaccharide derivative and an anticoagulant,
wherein the halogenated disaccharide derivative is present at 0.1-50 μM, and
wherein the thrombocytes are stabilized for a period of at least 6 hours.
2. The composition of claim 1 , wherein the composition further comprises a buffer.
3. The composition of claim 1 , wherein the anticoagulant is hirudin.
4. The composition of claim 1 , wherein the halogenated disaccharide derivative is selected from the group consisting of sucralose (1,6,-dichloro-1,6,dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-α-D-galactopyranoside), trichloronated maltose, 1,6,-dichloro-1,6,dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-6-O-monododecanoate-α-D-galactopyranoside, 1,6,-dichloro-1,6,dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-6-O-monotetradecanoate-α-D-galactopyranoside, and combinations thereof.
5. The composition of claim 1 , wherein the composition consists essentially of hirudin and sucralose (1,6,-dichloro-1,6,dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-α-D-galactopyranoside).
6. The composition of claim 1 , wherein the anticoagulant is selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), hirudin, heparin, and sodium citrate.