PATTERNED SURFACES
The present disclosure provides patterned materials that may be useful in reducing certain negative effects associated with damaged tissue in vivo. The patterned materials can modify the healing process, and may minimize the formation of scar tissue. Such effects can provide inhibition of adhesion between tissues and/or reduction of fibrotic encapsulation around implanted medical devices.
1 . A method for preventing or inhibiting the formation of scar tissue, comprising administering a patterned adhesion barrier comprising a base surface, wherein at least a portion of the base surface comprises a plurality of projections attached to the base surface and extending outward therefrom, wherein the projections are irregularly spaced with respect to each other and have an average length to diameter aspect ratio of at least about 5, in vivo, adjacent to one or more damaged tissues.
2 . The method of claim 1 , wherein the patterned adhesion barrier is administered at a surgical site within the abdominal, pelvic, cardiac, or spinal region.
3 . The method of claim 1 , wherein the patterned adhesion barrier is in the form of a freestanding film.
4 . A method for preventing or inhibiting the formation of fibrotic encapsulation of an medical device implanted within a body, comprising administering a patterned adhesion barrier comprising a base surface, wherein at least a portion of the base surface comprises a plurality of projections attached to the base surface and extending outward therefrom, wherein the projections are irregularly spaced with respect to each other and have an average length to diameter aspect ratio of at least about 5, in vivo, adjacent to the medical device.
5 . The method of claim 4 , wherein the administering step is performed at the same time as the medical device is implanted.
6 . The method of claim 4 , wherein the administering step is performed prior or subsequent to the time the medical device is implanted.
7 . The method of claim 4 , wherein the patterned adhesion barrier is in the form of a freestanding film.
8 . The method of claim 4 , wherein the patterned adhesion barrier is associated with the medical device prior to implantation.
9 . A method of decreasing collagen production, comprising administering a patterned adhesion barrier comprising a base surface, wherein at least a portion of the base surface comprises a plurality of projections attached to the base surface and extending outward therefrom, wherein the projections are irregularly spaced with respect to each other and have an average length to diameter aspect ratio of at least about 5, in vivo to one or more cells.
10 . The method of claim 9 , wherein the one or more cells express fibroblast genes and the patterned adhesion barrier reduces the expression of fibroblast genes in the cell as evidenced by a decrease in the amount of one or more of: TGFβ1 ligand, TβR2 receptor, or Smad3 intercellular mediator in the cell.
11 . The method of claim 10 , wherein the projections have an average length of at least about 5 μm and the patterned adhesion barrier reduces the expression of fibroblast genes in the cell by at least about 20% as compared with a comparable non-patterned material.
12 . The method of claim 10 wherein the projections have an average length of at least about 15 μm and the patterned adhesion barrier reduces the expression of fibroblast genes in the cell by at least about 50% as compared with a comparable non-patterned material.
13 . The method of claim 10 , wherein the projections have an average length to diameter aspect ratio of at least about 5 and the patterned adhesion barrier reduces the expression of fibroblast genes in the cell by at least about 20% as compared with a comparable non-patterned material.
14 . The method of claim 10 , wherein the projections have an average length to diameter aspect ratio of at least about 15 and the patterned adhesion barrier reduces the expression of fibroblast genes in the cell by at least about 50% as compared with a comparable non-patterned material.
15 . The method of claim 9 , wherein the one or more cells expresses fibroblast genes and wherein the patterned adhesion barrier leads to changes in fibroblast morphology as compared with fibroblast morphology observed by administering a comparable non-patterned material.
16 . The method of claim 15 , wherein the changes in fibroblast morphology are evidenced by a reduction in internal cellular tension.
17 . The method of claim 15 , wherein the changes in fibroblast morphology are evidenced by a reduced cell surface area.
18 . The method of claim 17 , wherein the cell surface area is reduced by at least about 50%.