REACTION METHOD, DETECTION METHOD, AND DETECTION DEVICE
In a first step, by moving a blocking liquid containing a blocking agent in a containing unit for containing a liquid, the inside of the containing unit is subjected to a blocking treatment. In a second step, by supplying a liquid containing a reaction target substance (detection target substance) into the containing unit, a reaction field which is exposed to the containing unit and to which a capturing body is fixed is caused to capture the reaction target substance. The second step is performed following the first step or simultaneously with the first step.
1 . A reaction method for bonding a capturing body to a reaction target substance in a container including a containing unit for containing a liquid and a reaction field which is exposed to the containing unit and to which the capturing body is fixed, the reaction method comprising:
a step of subjecting the inside of the containing unit to a blocking treatment by moving a blocking liquid containing a blocking agent in the containing unit; and
a step of causing the capturing body to capture the reaction target substance by supplying a liquid containing the reaction target substance into the containing unit, wherein
the step of causing the capturing body to capture the reaction target substance is performed following the step of performing the blocking treatment or simultaneously with the step of performing the blocking treatment.
2 . The reaction method according to claim 1 , wherein
a flow rate of a liquid in the containing unit is 1600 μL/min or more and 3300 μL/min or less in the step of performing the blocking treatment, and
a cross-sectional area of the containing unit in a cross section perpendicular to a flowing direction of the liquid on the reaction field is 0.01 mm 2 or more and 100 mm 2 or less.
3 . The reaction method according to claim 1 , wherein
the step of causing the capturing body to capture the reaction target substance is performed following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
4 . The reaction method according to claim 1 , wherein
the step of causing the capturing body to capture the reaction target substance is performed simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the liquid containing the reaction target substance in the containing unit is 2% by mass or more and 3% by mass or less.
5 . A detection method for detecting presence or the amount of a detection target substance in a specimen by utilizing surface plasmon resonance using a detection chip including a containing unit for containing a liquid and a metal film with a reaction field which is exposed to the containing unit and to which a capturing body is fixed, the detection method comprising:
a step of subjecting the inside of the containing unit to a blocking treatment by moving a blocking liquid containing a blocking agent in the containing unit;
a step of causing the capturing body to capture the detection target substance by supplying the specimen into the containing unit; and
a step of detecting a light quantity of fluorescence emitted from the vicinity of a surface of the metal film on a side of the containing unit or a light quantity of light reflected by the metal film by irradiating the metal film with light such that surface plasmon resonance occurs on the metal film while the detection target substance is bonded to the capturing body, wherein
the step of causing the capturing body to capture the detection target substance is performed following the step of performing the blocking treatment or simultaneously with the step of performing the blocking treatment.
6 . The detection method according to claim 5 , wherein
a flow rate of a liquid in the containing unit is 1600 μL/min or more and 3300 μL/min or less in the step of performing the blocking treatment, and
a cross-sectional area of the containing unit in a cross section perpendicular to a flowing direction of the liquid on the reaction field is 0.01 mm 2 or more and 100 mm 2 or less.
7 . The detection method according to claim 5 , wherein
the step of causing the capturing body to capture the detection target substance is performed following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
8 . The detection method according to claim 5 , wherein
the step of causing the capturing body to capture the detection target substance is performed simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the specimen in the containing unit is 2% by mass or more and 3% by mass or less.
9 . A detection device for detecting presence or the amount of a detection target substance in a specimen by utilizing surface plasmon resonance using a detection chip including a containing unit for containing a liquid and a metal film with a reaction field which is exposed to the containing unit and to which a capturing body is fixed, the detection device comprising:
a holder for holding the detection chip;
a liquid feeding unit for supplying a liquid into the containing unit while the detection chip is held by the holder;
a light irradiation unit for irradiating the metal film of the detection chip held by the holder with light such that surface plasmon resonance occurs on the metal film; and
a light detection unit for detecting a light quantity of fluorescence emitted from the vicinity of a surface of the metal film on a side of the containing unit or a light quantity of light reflected by the metal film when the light irradiation unit irradiates the metal film with light, wherein
the liquid feeding unit performs:
a step of subjecting the inside of the containing unit to a blocking treatment by supplying a blocking liquid containing a blocking agent into the containing unit and moving the blocking liquid in the containing unit; and
a step of causing the capturing body to capture the detection target substance by supplying the specimen into the containing unit, and
the liquid feeding unit performs the step of causing the capturing body to capture the detection target substance following the step of performing the blocking treatment or simultaneously with the step of performing the blocking treatment.
10 . The detection device according to claim 9 , wherein
a flow rate of a liquid moved by the liquid feeding unit in the containing unit is 1600 μL/min or more and 3300 μL/min or less, and
a cross-sectional area of the containing unit in a cross section perpendicular to a flowing direction of the liquid on the reaction field is 0.01 mm 2 or more and 100 mm 2 or less.
11 . The detection device according to claim 9 , wherein
the liquid feeding unit performs the step of causing the capturing body to capture the detection target substance following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
12 . The detection device according to claim 9 , wherein
the liquid feeding unit performs the step of causing the capturing body to capture the detection target substance simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the specimen in the containing unit is 2% by mass or more and 3% by mass or less.
13 . The reaction method according to claim 2 , wherein
the step of causing the capturing body to capture the reaction target substance is performed following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
14 . The reaction method according to claim 2 , wherein
the step of causing the capturing body to capture the reaction target substance is performed simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the liquid containing the reaction target substance in the containing unit is 2% by mass or more and 3% by mass or less.
15 . The detection method according to claim 6 , wherein
the step of causing the capturing body to capture the detection target substance is performed following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
16 . The detection method according to claim 6 , wherein
the step of causing the capturing body to capture the detection target substance is performed simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the specimen in the containing unit is 2% by mass or more and 3% by mass or less.
17 . The detection device according to claim 10 , wherein
the liquid feeding unit performs the step of causing the capturing body to capture the detection target substance following the step of performing the blocking treatment, and
a concentration of the blocking agent in the blocking liquid is 5% by mass or more and 8% by mass or less.
18 . The detection device according to claim 10 , wherein
the liquid feeding unit performs the step of causing the capturing body to capture the detection target substance simultaneously with the step of performing the blocking treatment, and
a concentration of the blocking agent in a mixed liquid of the blocking liquid and the specimen in the containing unit is 2% by mass or more and 3% by mass or less.