CARRIER FOR AFFINITY CHROMATOGRAPHY
Provided is an affinity chromatography carrier that suppresses nonspecific adsorption of impurities, suppresses a decrease in dynamic binding capacity due to long-term storage, and has high storage stability. The affinity chromatography carrier includes a solid support including a copolymer including (M-1) more than 20 parts by mass and 99.5 parts by mass or less of a structural unit derived from an epoxy group-containing monovinyl monomer with respect to and (M-2) 0.5 to 80 parts by mass of a structural unit derived from a polyvinyl monomer with respect to all structural units; ring-opened epoxy groups obtained by subjecting the epoxy groups to the ring-opening ; and a ligand coupled to the solid support, and is such that after a certain column vessel is packed with the affinity chromatography carrier and then purged with a 2 M NaCl-containing 20 mM sodium phosphate buffer with a pH of 7.5, allowing the column to stand at 40° C. for 7 days increases pH in the column by at most 2.
1 . An affinity chromatography carrier, the carrier comprising:
a solid support comprising a copolymer comprising:
(M-1) more than 20 parts by mass to 99.5 parts by mass or less of a structural unit derived from an epoxy group-containing monovinyl monomer with respect to all structural units and
(M-2) 0.5 to 80 parts by mass of a structural unit derived from a polyvinyl monomer with respect to all the structural units;
ring-opened epoxy groups obtained by subjecting the epoxy groups to the ring-opening; and
a ligand coupled to the solid support,
wherein pH in a column vessel packed with the carrier and then purged with a 2 M NaCl-containing 20 mM sodium phosphate buffer with a pH of 7.5 increases by at most 2 after the column stands at 40° C. for 7 days.
2 . The carrier according to claim 1 , further comprising: (M-3) 40 parts by mass or less of a structural unit derived from an epoxy-free monovinyl monomer with respect to all the structural units.
3 . The carrier according to claim 1 , wherein the ring-opened epoxy groups are represented by formula (1):
wherein R 1 represents a divalent organic group of 1 to 6 carbon atoms, R 2 represents a hydrogen atom or a monovalent organic group of 1 to 10 carbon atoms, X represents a thio group (>S), a sulfinyl group (>S═O), a sulfonyl group (>S(═O) 2 ), an imino group (>NH), or an oxy group (>O), and * represents a bonding position.
4 . The carrier according to claim 3 , wherein R 2 is represented by formula (2):
wherein R 3 represents a divalent or trivalent organic group of 1 to 10 carbon atoms, n represents 1 or 2, and ** represents a position of bonding with X in the formula (1).
5 . The carrier according to claim 3 , wherein X is a thio group (>S), a sulfinyl group (>S═O), or an oxy group (>O).
6 . The carrier according to claim 1 , wherein the ligand is an immunoglobulin-binding protein.
7 . The carrier according to claim 6 , wherein the immunoglobulin-binding protein is one or more selected from the group consisting of protein A, protein G, protein L, Fc-binding protein, and a functional variant thereof.
8 . The carrier according to claim 1 , wherein the solid support is a porous particle.
9 . The carrier according to claim 8 , wherein the porous particle has a particle size of 35 to 100 μm.
10 . An column, comprising:
a column vessel; and
the carrier according to claim 1 ,
wherein the column vessel is packed with the carrier.
11 . A method for purifying a target material, the method comprising:
purifying the target material by using the carrier according to claim 1 .
12 . The method according to claim 11 , wherein the target material is a target protein.