Device including a polymeric material for detecting species and stimulus and method of using thereof
A device for sensing and/or detecting species or stimulus at a locus, preferably at a fluid or moist locus by means of fluid communication therewith, comprising a surface configured to contact the locus, wherein the surface comprises a polyurethane material which material comprises a polyurethane polymer network having a hydrophilic polymer immobilised therein wherein the hydrophilic polymer comprises immobilised therein a ligand or moiety for detecting or sensing species or stimulus and an indicator for indicating detection or sensing prior to contact with the locus and change in indication as a function of species or stimulus present at the locus wherein species or stimulus are selected from chemical and biological species or stimulus; the use thereof preferably for detecting and/or sensing or binding bacteria, or for detecting or sensing pH or for detection or scanning with use of a reader; and a Kit.
1. A device for detecting a microbe or a pH change due to the presence of a microbe at a locus comprising a polymeric material, wherein the polymeric material comprises:
a semi-interpenetrating network of a polyurethane and a hydrophilic polymer, wherein the hydrophilic polymer comprises a polyacrylamide or a copolymer thereof;
a first moiety comprising an antibiotics or a derivative of antibiotics modified to be devoid of antibiotic activity configured to bind the microbe, or an acid group that becomes charged at a different pH, wherein the hydrophilic polymer is configured to change from a coiled configuration to a globular configuration in response to the binding of the microbe or the charge on the acid group;
a second moiety as an indicator comprising a solvatochromic dye configured to change color in response to the hydrophilic polymer being changed from the coiled configuration to the globular configuration, wherein the second moiety is different from the first moiety; and
wherein the first moiety and the second moiety are covalently bonded to the hydrophilic polymer.
2. The device of claim 1 , wherein the microbe is selected from the group consisting of bacteria, yeast, fungus, and combinations thereof.
3. The device of claim 1 , further comprising a locus-contacting surface, wherein the locus-contacting surface comprises the polymeric material.
4. The device of claim 1 , further comprising a locus-contacting surface and an opposing non-locus-contacting surface, wherein one of the locus-contacting surface and the opposing non-locus-contacting surface comprises the polymeric material; or both of the locus-contacting surface and the opposing non-locus-contacting surface comprise the polymeric material.
5. The device of claim 1 , wherein the polymeric material is fluid permeable.
6. The device of claim 1 , wherein the device is configured to be activated by fluid contact.
7. The device of claim 1 , wherein the hydrophilic polymer is a copolymer of a hydrophilic monomer and an indicating monomer.
8. The device of claim 1 , wherein said first moiety and said second moiety are immobilised in the semi-interpenetrating network and are not leached from the device.
9. The device of claim 1 , wherein the change of color is configured to indicate the detection of the microbe or the pH change due to the presence of the microbe by means of a change in fluorescence, or wavelength, or intensity thereof.
10. The device of claim 1 , wherein the locus comprises an exuding or humid wound environment.
11. The device of claim 1 , wherein the polymeric material is in the form of foam, or of a film, perforated film, membrane, water impermeable membrane providing moisture vapour transmission (MVT), adhesive layer or coating, sheet, block, non-woven or woven fabric, thread, ribbon or combinations thereof.
12. The device of claim 1 , wherein the hydrophilic polymer comprises a polymer selected from the group consisting of polyacrylamide, polyalkyl acrylamide, polyallyl acrylamide, wherein alkyl is ethyl, propyl or butyl.
13. The device of claim 1 , further comprising a wound dressing, a device for interrogation of wound fluid, a medical or dental sponge or wipe, or pH sensor.
14. The device of claim 1 , further comprising an inspection or scanning device or reader configured to receive information relating to color or change in color and provide output information,
wherein the inspection or scanning device or reader comprises: interrogation means for acquiring indicating information; a processor for processing indicating information and generating output information; and a display or connectivity for a display for displaying output information.
15. A kit for detecting a microbe or a pH change due to the presence of a microbe at an wound, comprising the device of claim 14 and a reference strip providing reference information for processing information relating to color and change in color and generating detection information.
16. A method for detecting a microbe or a pH change due to the presence of a microbe at a locus comprising:
providing the device of claim 1 ;
applying the device of claim 1 in or on the locus; and
detecting a change in color of the polymeric material.
17. The method of claim 16 , wherein detecting the change in color of the polymeric material comprises providing an inspection or scanning device or reader; acquiring indication information for processing; and displaying or transmitting for display output information.
18. The method of claim 16 , further comprising quantifying or classifying the change in color.
19. A method for detecting a microbe or a pH change due to the presence of a microbe, comprising:
providing a device comprising a wound contacting surface, wherein the wound contacting surface comprises a polymeric material, wherein the polymeric material comprises:
a semi-interpenetrating network of a polyurethane and a hydrophilic polymer, wherein the hydrophilic polymer comprises a polyacrylamide or a copolymer thereof:
a first moiety comprising an antibiotics or a derivative of antibiotics modified to be devoid of antibiotic activity configured to bind the microbe, or an acid group that becomes charged at a different pH, wherein the hydrophilic polymer is configured to change from a coiled configuration to a globular configuration in response to the detection of the microbe or the change on the acid group;
a second moiety as an indicator comprising a solvatochromic dye configured to change color in response to the hydrophilic polymer being changed from the coiled configuration to the globular configuration, wherein the second moiety is different from the first moiety; and
wherein the first moiety and the second moiety and the second moiety are covalently bonded to the hydrophilic polymer; applying the wound contacting surface in or on the wound; and detecting a change in color in the indicator.
20. The device of claim 1 , wherein the second moiety comprises one or more selected from a group consisting of: Nile Red, Nile Blue, Dansyl cadavarine Dansyl chloride, DANSAEP, 1-hydroxy-4-[4[hydroxyethylsulphonyl)-phenylazo]-napthalene-2-sulphonate, 2-fluoro-4-[4[(2-hydroxyethanesulphonyl)-phenylazo]-6-methoxy phenol, 4-[4-(2-hydroxyethylsulphonyl)-phenylazo]-2,6-dimethoxyphenol, 4-(2-acryloyl oxyethylamino)-7-nitro-2,1,3-benzoxadiazole (NBD-AE), 4-(2-acryloylaminoethylamino)-7-nitro-2,1,3-benzodiazole (NBD-AA), 2-(6-(dimethylamino)-1,3-dioxo-1H-benzo(de)isoquinolin-2(3H)-yl) ethyl methacrylate and derivatives and combinations thereof.
21. The device of claim 12 , wherein the hydrophilic polymer comprises poly(N-isopropyl acrylamide).
22. The device of claim 1 , wherein the first moiety comprises a carboxylic acid group, a succinimide group, vancomycin, polymyxin, beta-lactam, teicoplanin antibiotics, cecropin and melittin hybrid, CEME, defensins or a derivative thereof modified to be devoid of antibiotic activity.
23. The device of claim 12 , wherein the hydrophilic polymer comprises poly(N-isopropyl acrylamide).