Anti-inflammatory properties of a surface protein of propionibacterium freudenreichii
View Patent ↗Disclosed is a polypeptide including or consisting of the amino acid sequence SEQ id n o :1, for use in the treatment or prevention of an inflammatory disease. It further encompasses a nucleic acid sequence encoding a polypeptide, a vector including a nucleic acid and a host cell including a nucleic acid sequence and/or a vector, for use in the treatment or prevention of an inflammatory disease. Also disclosed is a pharmaceutical composition or a food composition including a polypeptide, a nucleic acid sequence, a vector or a host cell and a pharmaceutically acceptable carrier or a dairy product, for the treatment of inflammatory disease. Additionally, a method for the screening of bacteria having immunomodulatory properties is disclosed.
1. A method for treating or preventing an inflammatory disease in a patient, comprising administering to a patient in need thereof a therapeutically effective amount of a polypeptide comprising or consisting of an amino acid sequence of a surface layer protein B (SlpB) of Propinibacterium freudenreichii or fragment thereof consisting of a processed SlpB protein of Propinibacterium freudenreichii devoid of a signal peptide.
2. The method according to claim 1 , wherein said inflammatory disease is an inflammatory bowel disease selected from the group consisting of Crohn's disease, ulcerative colitis, ileitis, pouchitis and enteritis.
3. The method according to claim 1 , wherein said fragment consists of amino acid 30 to 556 of SEQ ID NO: 1 corresponding to the 527 residues of processed SlpB devoid of the 29 residues of a long signal peptide.
4. The method according to claim 1 , wherein the polypeptide is in a pharmaceutical composition comprising a pharmaceutically acceptable carrier.
5. The method of claim 1 , wherein the SlpB of Propinibacterium freudenreichii is selected from the group consisting of an amino acid sequence of SEQ ID NO: 1 and amino acid sequences having at least 95% of identity with SEQ ID NO: 1and having anti-inflammatory properties of SEQ ID NO: 1.
6. The method of claim 1 , wherein the SlpB of Propinibacterium freudenreichii is selected the group consisting of an amino acid sequence of SEQ ID NO: 1 and amino acid sequences having at least 99% of identity with SEQ ID NO: 1 and having anti-inflammatory properties of SEQ ID NO: 1 .
7. The method of claim 1 , wherein the SlpB of Propinibacterium freudenreichii is the amino acid sequence of SEQ ID NO: 1.
8. The method of claim 7 , wherein said method is for treating for an inflammatory disease in a patient in need thereof.