Compositions and methods for increasing plant growth and yield using rice promoters
Compositions and methods for improving plant growth are provided herein. Compositions comprise promoter sequences that direct expression of an operably linked nucleotide in a developmentally regulated manner. Polynucleotides, polypeptides, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method of expressing a gene of interest in a plant comprising transforming a plant cell with a DNA construct comprising a promoter sequence, wherein said promoter sequence comprises the nucleic acid sequence set forth in SEQ ID NO: 3, and wherein said promoter sequence is operably linked to a second nucleic acid sequence encoding a functional protein, and regenerating a transformed plant.
2. The method of claim 1 , wherein said second nucleic acid sequence encodes a protein involved in photosynthesis.
3. The method of claim 1 , wherein said second nucleic acid encodes an enzyme with sedoheptulose-1,7-bisphosphatase activity.
4. The method of claim 3 , wherein said second nucleic acid comprises the sequence of SEQ ID NO: 1 or a sequence having at least 90% sequence identity to the sequence of SEQ ID NO: 1.
5. The method of claim 3 , wherein said nucleic acid sequence encodes the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO: 2.
6. The method of claim 1 where the transformed plant is a monocotyledonous plant.
7. The method of claim 1 where the transformed plant is a dicotyledonous plant.
8. A DNA construct comprising, in operable linkage,
a. a promoter that functions in the plant cell,
b. a nucleic acid sequence encoding a protein involved in photosynthesis,
wherein said promoter comprises the nucleotide sequence set forth in SEQ ID NO: 3, and wherein said promoter is heterologous to said nucleic acid sequence encoding a protein involved in photosynthesis.
9. The DNA construct of claim 8 , wherein the nucleic acid sequence encoding a protein involved in photosynthesis comprises the nucleic acid sequence of SEQ ID NO: 1.
10. The DNA construct of claim 8 , wherein the nucleic acid sequence encoding a protein involved in photosynthesis encodes the amino acid sequence of SEQ ID NO: 2.
11. A transformed plant comprising a DNA construct comprising a promoter that functions in a plant cell, wherein said promoter comprises the sequence set forth in SEQ ID NO: 3, and wherein said promoter is operably linked to a nucleic acid sequence encoding a protein involved in photosynthesis, and wherein said promoter is heterologous to said nucleic acid sequence encoding a protein involved in photosynthesis.
12. The transformed plant of claim 11 wherein said protein involved in photosynthesis is encoded by the sequence of SEQ ID NO: 1.
13. The transformed plant of claim 11 wherein said protein involved in photosynthesis comprises the sequence of SEQ ID NO: 2.
14. A method of modulating the growth of a plant comprising inserting into the nuclear genome of a plant cell a promoter sequence upstream of a photosynthetic gene of interest to alter the expression of a said photosynthetic gene, wherein said promoter sequence comprises the sequence set forth in SEQ ID NO: 3.
15. The method of claim 14 in which the photosynthetic gene encodes a protein with sedoheptulose-1,7-bisphosphatase activity.
16. A DNA construct comprising a promoter sequence, said promoter sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 3, wherein said promoter sequence is operably linked to a nucleotide of interest encoding a protein involved in photosynthesis and wherein said promoter sequence and said nucleotide of interest are heterologous to each other.
17. The DNA construct of claim 16 , wherein said nucleotide of interest encodes an enzyme having sedoheptulose-1,7-bisphosphatase activity.
18. The DNA construct of claim 17 , wherein said enzyme having sedoheptulose-1,7-bisphosphatase activity shares at least 95% identity with the amino acid sequence set forth in SEQ ID NO:2.
19. Transformed seed of the plant of claim 11 .