Protein with dextran-saccharase activity, and uses
View Patent ↗The invention relates to: —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; —a complex containing a substrate and a protein with dextran-saccharase activity —a method for synthesizing dextrans; and —dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.
1. A process for synthesizing dextrans comprising:
forming dextrans by contacting sucrose in a synthesis medium with
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1.
2. A process for synthesizing dextrans comprising:
forming dextrans by contacting sucrose in a synthesis medium with
(a) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1.
3. The process of claim 1 , wherein the step of contacting to form dextrans is carried out either at a temperature of between 20° C. and 50° C., or at a temperature of between 25° C. and 45° C.
4. The process of claim 1 , wherein the sucrose is present in the synthesis medium at either a concentration of between 50 and 600 g·l −1 , or a concentration of between 50 and 400 g·l −1 .
5. The process of claim 1 , wherein the step of contacting to form dextrans takes place either at a pH that is between 4 and 7, or at a pH of approximately 5.75.
6. A process for synthesizing glucooligosaccharides comprising:
forming glucooligosaccharides by contacting a synthesis medium that comprises sucrose and at least one carbohydrate acceptor with
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1.
7. A process for synthesizing glucoconjugate compound comprising:
forming glucoconjugate compounds by contacting synthesis medium that comprises sucrose and at least one hydroxylated molecule with
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1 and position 563 to 1282 within SEQ ID NO: 1.
8. A process for synthesizing glucooligosaccharides comprising:
(i) forming glucooligosaccharides by contacting a synthesis medium that comprises sucrose and at least one carbohydrate acceptor with
(a) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1.
9. A process for synthesizing glucoconjugate compounds comprising:
forming a glucoconjugate compounds by contacting a synthesis medium that comprises sucrose and at least one hydroxylated molecule with
(a) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 1 or
(b) a complex comprising a support and a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 1,
wherein said protein with dextransucrase activity has been immobilized on said support.
10. The process of claim 1 , wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
11. The process of claim 10 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.
12. The process of claim 2 , wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
13. The process of claim 12 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.
14. The process of claim 6 , wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
15. The process of claim 14 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.
16. The process of claim 7 , wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
17. The process of claim 16 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.
18. The process of claim 8 wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
19. The process of claim 18 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.
20. The process of claim 9 , wherein the protein with dextransucrase activity is selected from
(a) a protein with dextransucrase activity where the protein is at least 95% identical to SEQ ID NO: 2 or
(b) a protein with dextransucrase activity where the protein is at least 98% identical to SEQ ID NO: 2.
21. The process of claim 20 , wherein the protein with dextransucrase activity comprises the amino acid sequence set forth in SEQ ID NO: 2.