Recombinant fibrinogen

1. A nucleotide sequence which is optimized for expression in a mammalian cell culture system, comprising:

(i) a nucleotide sequence according to SEQ ID NO. 4 or 7, or a part thereof which comprises nucleotides 60 to 1932 of SEQ ID NO. 4, nucleotides 60 to 1887 of SEQ ID NO. 4 or nucleotides 60-2598 of SEQ ID NO. 7, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 4 or 7 and which encodes a fibrinogen alpha chain, or

(ii) a nucleotide sequence according to SEQ ID NO. 5, or the part thereof which comprises nucleotides 93 to 1473 of SEQ ID NO. 5, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 5 and which encodes a fibrinogen beta chain or

(iii) a nucleotide sequence according to SEQ ID NO. 6 or 12, or a part thereof which comprises nucleotides 81 to 1311 of SEQ ID NO. 6 or nucleotides 81 to 1359 of SEQ ID NO. 12, or a nucleotide sequence which has a sequence which is at least 90% identical to SEQ ID NO. 6 or 12 and which encodes a fibrinogen gamma chain.

2. The nucleotide sequence of claim 1 , wherein the nucleotide sequence is optimized for expression in a COS cell, a BHK cell, a NSO cell, a CHO cell, a SP2/0 or a human cell culture system.

3. A nucleotide sequence according to claim 1 which is optimized for expression in a PER.C6 cell or a HEK293 cell culture system.

4. The nucleotide sequence of claim 1 , wherein the nucleotide sequence has a codon adaption index of at least 0.95.

5. The nucleotide sequence of claim 1 , wherein the nucleotide sequence has a GC content of at least 55%.

6. The nucleotide sequence of claim 1 , wherein the nucleotide sequence contains no cis-acting sites.

7. A nucleotide construct comprising the nucleotide sequence of claim 1 .

8. A mammalian cell comprising the nucleotide sequence of claim 1 .