Cartridges, kits, and methods for enhanced detection and quantification of analytes
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
1. A microfluidic cartridge comprising:
a reservoir configured to hold a fluid, the reservoir further configured to receive a sample from a sample collection device, the reservoir comprising an outlet having a phase-changeable material therein to occlude an entire cross-section of the outlet; and
a circuit board comprising:
a sensor configured to generate a signal indicative of at least one of a presence, absence, or quantity of one or more analytes within the sample;
circuitry configured to be activated by insertion of the sample collection device;
a temperature sensor positioned on the circuit board to sense temperature indicative of temperature of the fluid in the reservoir; and
one or more heaters, wherein at least one of the one or more heaters is configured to heat the phase-changeable material such that the phase-changeable material does not occlude the entire cross-section of the outlet.
2. The microfluidic cartridge of claim 1 , wherein the circuit board further comprises one or more spring contacts coupled to a piezoelectric transducer.
3. The microfluidic cartridge of claim 2 , wherein the piezoelectric transducer is configured to emit energy into the reservoir to mix the fluid, the sample, and reagents.
4. The microfluidic cartridge of claim 3 , wherein the piezoelectric transducer forms at least a part of a wall of the reservoir.
5. A microfluidic cartridge comprising:
a reservoir configured to hold a fluid, the reservoir further configured to receive a sample from a sample collection device;
a piezoelectric transducer configured to emit energy into the reservoir to mix the fluid, the sample, and reagents, wherein the piezoelectric transducer is adhered to the reservoir to form at least a part of a wall of the reservoir using an annulus of adhesive applied on a top surface of the piezoelectric transducer to permit fluidic sealing between the piezoelectric transducer and the reservoir while permitting the piezoelectric transducer to vibrate; and
a circuit board comprising:
one or more spring contacts coupled to the piezoelectric transducer;
a sensor configured to generate a signal indicative of at least one of a presence, absence, or quantity of one or more analytes within the sample;
circuitry configured to be activated by insertion of the sample collection device; and
a temperature sensor positioned on the circuit board to sense temperature indicative of temperature of the fluid in the reservoir.
6. The microfluidic cartridge of claim 3 , wherein the piezoelectric transducer is configured to emit energy in a modified manner based on the sensed temperature.
7. The microfluidic cartridge of claim 1 , wherein the circuit board forms a wall of an analysis channel such that the sensor is positioned in the analysis channel, and
wherein the analysis channel is configured to receive the fluid and the sample from the reservoir.
8. The microfluidic cartridge of claim 1 , wherein the sensor comprises a target working electrode, further comprising a positive control working electrode comprising affinity molecules pre-bound to the positive control working electrode, the positive control working electrode configured to generate a control signal based on a reaction between signaling agents directly or indirectly bound to the affinity molecules and a substrate,
wherein the target working electrode is configured to generate the signal indicative of at least one of the presence, absence, or quantity of the one or more analytes within the sample, and
wherein a test is determined to be reliable if the control signal indicates a quantity of the reaction within a predetermined range.
9. The microfluidic cartridge of claim 8 , further comprising a negative control working electrode comprising a self-assembled monolayer, the negative control working electrode configured to generate a negative control signal based on a reaction between the substrate and any signaling agents localized at the negative working electrode.
10. The microfluidic cartridge of claim 9 , wherein the negative control signal is indicative of reliability of the test.
11. The microfluidic cartridge of claim 1 , wherein the circuitry comprises a contact switch configured to send a signal indicative of insertion of the sample collection device within the microfluidic cartridge.
12. The microfluidic cartridge of claim 9 , wherein the sensor further comprises a reference electrode and a counter electrode.
13. The microfluidic cartridge of claim 8 , wherein the target working electrode is masked with a plurality of striations configured to promote homogenous distribution of a plurality of magnetic particles localized over the target working electrode and to promote resistance to movement of the plurality of magnetic particles off of the target working electrode.
14. The microfluidic cartridge of claim 8 , wherein the target working electrode comprises affinity molecules pre-bound to the working electrode.
15. The microfluidic cartridge of claim 1 , wherein the one or more analytes within the sample comprise one or more nucleic acids, and
wherein the reservoir is configured to permit amplification of the one or more nucleic acids within the sample.
16. The microfluidic cartridge of claim 1 , wherein the circuit board further comprises a fluidic isolator comprising phase-changeable material,
wherein at least one of the one or more heaters is configured to heat phase-changeable material of the fluidic isolator after unsealing the reservoir outlet such that phase-changeable material of the fluidic isolator flows into an analysis channel to fluidicly isolate the reservoir from a substrate reservoir.
17. The microfluidic cartridge of claim 1 , wherein at least one of the one or more heaters is configured to be heated by a resistor in the circuit board that is not electrically coupled to the at least one heater.
18. The microfluidic cartridge of claim 1 , wherein the microfluidic cartridge is sized to be at least partially disposed within, and electrically coupled via a plurality of leads on the circuit board to, a reader configured to receive and process the signal indicative of at least one of the presence, absence, or quantity of the one or more analytes within the sample.
19. A circuit board for a microfluidic cartridge configured to receive a sample from a sample collection device, the circuit board comprising:
a sensor configured to generate a signal indicative of at least one of a presence, absence, or quantity of one or more analytes within the sample;
circuitry configured to be activated by insertion of the sample collection device;
a temperature sensor positioned on the circuit board to sense temperature indicative of temperature of fluid in a reservoir; and
one or more heaters configured to heat phase-changeable material to open one or more valves.
20. The circuit board of claim 19 , wherein the circuit board comprises one or more spring contacts coupled to a piezoelectric transducer.
21. The circuit board of claim 19 , wherein the circuit board forms a wall of an analysis channel such that the sensor is positioned in the analysis channel.
22. The circuit board of claim 19 , wherein the sensor comprises a target working electrode, further comprising a positive control working electrode comprising affinity molecules pre-bound to the positive control working electrode, the positive control working electrode configured to generate a control signal based on a reaction between signaling agents directly or indirectly bound to the affinity molecules and a substrate,
wherein the target working electrode is configured to generate the signal indicative of at least one of the presence, absence, or quantity of the one or more analytes within the sample, and
wherein a test is determined to be reliable if the control signal indicates a quantity of the reaction within a predetermined range.
23. The circuit board of claim 22 , further comprising a negative control working electrode comprising a self-assembled monolayer, the negative control working electrode configured to generate a negative control signal based on a reaction between the substrate and any signaling agents localized at the negative working electrode, and
wherein the negative control signal is indicative of reliability of the test.
24. The circuit board of claim 19 , wherein the circuitry comprises a contact switch configured to send a signal indicative of insertion of the sample collection device within the microfluidic cartridge.
25. A circuit board for a microfluidic cartridge configured to receive a sample from a sample collection device, the circuit board comprising:
a sensor configured to generate a signal indicative of at least one of a presence, absence, or quantity of one or more analytes within the sample, the sensor comprising a target working electrode masked with a plurality of striations configured to promote homogenous distribution of a plurality of magnetic particles localized over the target working electrode and to promote resistance to movement of the plurality of magnetic particles off of the target working electrode.
26. The circuit board of claim 22 , wherein the target working electrode comprises a self-assembled monolayer or affinity molecules pre-bound to the target working electrode or both.
27. The circuit board of claim 19 , further comprising a fluidic isolator comprising phase-changeable material, wherein at least one of the one or more heaters is configured to heat phase-changeable material of the fluidic isolator after unsealing an outlet of a sample preparation reservoir such that phase-changeable material of the fluidic isolator flows into an analysis channel to fluidicly isolate the sample preparation reservoir from a substrate reservoir.
28. The circuit board of claim 19 , wherein at least one of the one or more heaters is configured to be heated by a resistor in the circuit board that is not electrically coupled to the at least one heater.
29. The circuit board of claim 25 , further comprising a positive control working electrode comprising affinity molecules pre-bound to the positive control working electrode, the positive control working electrode configured to generate a control signal based on a reaction between signaling agents directly or indirectly bound to the affinity molecules and a substrate,
wherein the target working electrode is configured to generate the signal indicative of at least one of the presence, absence, or quantity of the one or more analytes within the sample, and
wherein a test is determined to be reliable if the control signal indicates a quantity of the reaction within a predetermined range.
30. The circuit board of claim 25 , further comprising a negative control working electrode comprising a self-assembled monolayer, the negative control working electrode configured to generate a negative control signal based on a reaction between the substrate and any signaling agents localized at the negative working electrode, and
wherein the negative control signal is indicative of reliability of the test.