Provided is the use of or compositions or formulations comprising Burkholderia species, filtrate, supernatant, extract, pesticidally active compound or metabolite derived therefrom as an insecticide, particularly against infestation of Corn Rootworm larvae, and/or as crop yield enhancer.
1. A method for modulating infestation of Diabrotica (corn rootworm) in a location comprising
applying an effective amount of
(a) a supernatant, filtrate, and/or extract collected from a whole cell broth of Burkholderia A396 (NRRL Accession No. B-50319) fermentation, and
(b) at least one of a carrier, diluent or adjuvant,
to modulate said infestation of corn rootworm at said location.
2. The method according to claim 1 , wherein the location is on a plant, plant seed, plant roots, plant part, seedling or substrate for growing said plant.
3. The method according to claim 1 wherein said Diabrotica (corn rootworm) is Western or Northern Corn Rootworm.
4. The method according to claim 1 , wherein said Diabrotica (corn rootworm) is modulated by modulating the mortality of said corn rootworm.
5. The method according to claim 1 , wherein the mortality of Diabrotica (corn rootworm) is modulated and wherein there is a mortality of corn rootworm of at least about 50% at said location.
6. The method according to claim 1 , which further comprises applying another natural or artificial insecticidal substance.
7. A method for modulating plant yield and/or Diabrotica infestation comprising
applying an effective amount of a supernatant, filtrate, and/or extract collected from a whole cell broth of Burkholderia A396 (NRRL Accession No. B-50319) fermentation to a plant or plant part.
8. The method of claim 7 , wherein the plant part is a seed.
9. A method to produce a Diabrotica resistant plant seed comprising the step of
applying a supernatant, filtrate, and/or extract collected from a whole cell broth of Burkholderia A396 (NRRL Accession No. B-50319) fermentation to a plant seed.