EXTRACELLULAR MATRIX COMPOSITIONS
Provided herein are placental extracellular matrix (ECM) compositions and methods of making the same. Also provided herein are uses of the placental ECM compositions provided herein.
1 . An extracellular matrix (ECM) composition comprising collagen and elastin prepared from a chorionic plate that has been separated from a placenta.
2 . The composition of claim 1 , wherein said composition comprises less than 0.1% fibronectin.
3 . The composition of claim 1 , wherein said composition comprises an undetectable amount of laminin.
4 . The composition of claim 1 , wherein said composition comprises an undetectable amount of glycosaminoglycans.
5 . The composition of claim 1 , wherein said composition comprises less than 0.1% fibronectin, an undetectable amount of laminin, and an undetectable amount of glycosaminoglycans.
6 . The composition of claim 1 , wherein the composition comprises about 30-72% collagen and about 10-30% elastin.
7 . The composition of claim 6 , wherein said composition comprises less than about 0.1% fibronectin.
8 . The composition of claim 6 , wherein said composition comprises less than about 0.1% laminin.
9 . The composition of claim 6 , wherein said composition comprises less than about 0.1% glycosaminoglycans.
10 . The composition of claim 6 , wherein said composition comprises an undetectable amount of cytokines and growth factors.
11 . The composition of claim 6 , wherein said composition comprises less than about 0.1% fibronectin, less than about 0.1% laminin, less than about 0.1% glycosaminoglycans, and an undetectable amount of cytokines and growth factors.
12 - 13 . (canceled)
14 . The composition of claim 11 , wherein said placenta is a human placenta.
15 . The composition of claim 1 , wherein said composition is formulated as a sheet.
16 . (canceled)
17 . The composition of claim 1 , wherein said composition is formulated as a particulate.
18 . The composition of claim 1 , wherein said composition is formulated as a flowable matrix.
19 - 55 . (canceled)
56 . The composition of claim 1 , wherein the composition comprises about 30-72% collagen.
57 . The composition of claim 1 , wherein the composition comprises at least about 60% type I collagen.
58 . The composition of claim 1 , wherein the composition comprises about 10-35% elastin.
59 . The composition of claim 58 , wherein the composition comprises about 10-20% elastin.
60 . The composition of claim 1 , wherein the composition is decellularized.
61 . The composition of claim 17 , wherein the particulate is packaged in a suitable container.
62 . The composition of claim 61 , wherein the container contains about 200-300 mg, 100-200 mg, 150-250 mg, 50-100 mg, 25-50 mg, 10-25 mg, 5-10 mg, or 1-5 mg of the particulate.
63 . The composition of claim 61 , further comprising a pharmaceutically or cosmetically acceptable carrier that is added to the particulate prior to use.
64 . The composition of claim 18 , wherein the flowable matrix comprises 200-300 mg/ml, 100-200 mg/ml, 150-250 mg/ml, 0.1-100 mg/ml, 1-75 mg/ml, 1-50 mg/ml, 1-40 mg/ml, 10-40 mg/ml, or 20-40 mg/ml of ECM.
65 . The composition of claim 61 , wherein the container is a syringe.
66 . The composition of claim 15 , wherein the composition comprises a laminate comprising two or more sheets.
67 . The composition of claim 66 , wherein the laminate comprises one or more ECM sheets and a sheet that comprises a different planar decellularized tissue or a planar artificial tissue substitute.
68 . The composition of claim 66 , wherein the laminate comprises one or more ECM sheets.