Spatial zoom mode for accumulative trapped ion mobility spectrometry
View Patent ↗The invention relates to the operation of ion mobility spectrometers based on gases pushing the ions over electrical field barriers, preferably in combination with mass spectrometers, and relates to trapped ion mobility spectrometers (“TIMS”). The invention proposes to accumulate and to scan the ions of a selected range of mobilities by using a long and flat electric field ramp created by additional voltages. By a voltage supplied at the beginning of the flat ramp, the lowest mobility of the mobility range of ions to be collected can be selected. By the difference of the voltages at the beginning and the end, the width of the mobility range is determined. The spatial zoom advantageously can collect considerable more ions of interest than a temporal zoom without severe losses by space charge effects, and more ions can be detected in the mass-mobility map.
1. A method for analyzing ions of a selected range of mobilities using a trapped ion mobility separator comprising an accumulation unit and a scan unit, comprising the steps of:
(a) collecting ions over a range of mobilities in the accumulation unit;
(b) transferring ions from the accumulation unit into the scan unit wherein a voltage of the scan unit is adjusted such that transferred ions having a mobility lower than a first value are not stored in the scan unit and transferred ions having a mobility higher than the predetermined value are stored in the scan unit; and
(c) separating the stored ions of the selected mobility range according to mobility by scanning a voltage of the scan unit.
2. The method according to claim 1 , wherein, in the step (b), a voltage of the accumulation unit is adjusted such that ions which are collected in the accumulation unit and have a mobility higher than a second value, said second value being higher than the first value, remain stored in the accumulation unit, and wherein the remaining ions are neutralized or ejected from the accumulation unit.
3. The method according to claim 2 , wherein ions are neutralized by switching off an RF voltage applied to the accumulation unit for radially confining ions during steps (a) and (b).
4. The method according to claim 1 , wherein ions which have a mobility higher than a second value, said second value being higher than the first value, are filtered out before entering the accumulation unit.
5. The method according to claim 1 , wherein an additional voltage at or near an entrance of the scan unit is adjusted such that the ions of the selected range of mobilities spread over the full length of the scan unit to reduce space charge effects.
6. The method according to claim 1 , wherein the separated ions are analyzed by a downstream mass analyzer.
7. The method according to claim 1 , wherein the separated ions are fragmented to produce fragment ions that are analyzed by a downstream mass analyzer.
8. The method according to claim 1 , wherein the separated ions pass a band-pass mass filter that produces filtered ions that are then fragmented, and resulting fragment ions are analyzed by a downstream mass analyzer.
9. The method according to claim 1 , wherein an inflow of ions into the accumulation unit is stopped during the step of transferring.
10. The method according to claim 9 , wherein the inflow of ions into the accumulation unit is stopped by adjusting a DC voltage at, near or upstream of the accumulation unit.
11. The method according to claim 1 , further comprising repeating steps (a) to (c) with different ranges of mobilities by adjusting the voltage of the scan unit.
12. The method according to claim 2 , further comprising repeating steps (a) to (c) with different ranges of mobilities by adjusting the voltages of the scan unit and the accumulation unit.
13. The method according to claim 3 , further comprising repeating steps (a) to (c) with different ranges of mobilities by adjusting the voltages of the scan unit and the accumulation unit.
14. A method for analyzing ions of a selected range of mobilities using a trapped ion mobility separator comprising an accumulation unit and a scan unit, comprising the steps of:
(a) collecting ions over a range of mobilities in the accumulation unit while ions in the scan unit are separated according to mobility by scanning a voltage of the scan unit;
(b) transferring ions from the accumulation unit into the scan unit after the scan of step (a) is finished wherein a voltage of the scan unit is adjusted such that transferred ions having a mobility lower than a first value are not stored in the scan unit and transferred ions having a mobility higher than the first value are stored in the scan unit; and
(c) separating the ions stored in the scan unit according to mobility by scanning a voltage of the scan unit.