Microbiota restoration therapy (MRT), compositions and methods of manufacture
Microbiota restoration therapy compositions and methods for manufacturing, processing, and/or delivering microbiota restoration therapy compositions are disclosed. An example method may include a method of treating an infection. The method may comprise administering a microbiota restoration therapy composition to a patient with an infection. The microbiota restoration therapy composition may comprise a mixture of an effective amount of fecal microbiota and an effective amount of polyethylene glycol.
1. A method of treating a drug-resistant infection, the method comprising:
administering a microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection; and
wherein the microbiota restoration therapy composition comprises a mixture of an effective amount of fecal microbiota from a fresh human fecal sample from a human donor and polyethylene glycol at a concentration of 30-90 g/L.
2. The method of claim 1 , wherein the antibiotic-resistant bacterial infection includes a vancomycin-resistant enterococci infection.
3. The method of claim 1 , wherein the antibiotic-resistant bacterial infection includes a methicillin-resistant Staphylococcus aureus infection.
4. The method of claim 1 , wherein the antibiotic-resistant bacterial infection includes a multi-drug resistant bacterial infection.
5. The method of claim 1 , further comprising administering a second dose of the microbiota restoration therapy composition to the patient.
6. The method of claim 1 , further comprising manufacturing the microbiota restoration therapy composition and wherein manufacturing the microbiota restoration therapy composition comprises:
collecting the fresh human fecal sample;
adding the polyethylene glycol to the fresh human fecal sample to form a diluted sample;
mixing the diluted sample with a mixing apparatus;
filtering the diluted sample;
wherein filtering forms a filtrate;
transferring the filtrate to a sample bag; and
sealing the sample bag.
7. The method of claim 1 , wherein administering a microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection includes administering the microbiota restoration therapy composition with an enema tube.
8. The method of claim 1 , wherein administering a microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection includes orally administering the microbiota restoration therapy composition.
9. A method of treating an antibiotic-resistant bacterial infection, the method comprising:
manufacturing a microbiota restoration therapy composition, wherein manufacturing the microbiota restoration therapy composition comprises collecting a fresh human fecal sample and adding a mixture of polyethylene glycol at a concentration of 30 g/L in saline to the fresh human fecal sample;
administering a primary dose of the microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection;
administering a secondary dose of the microbiota restoration therapy composition to the patient with the antibiotic-resistant bacterial infection; and
wherein the antibiotic-resistant bacterial infection includes a vancomycin-resistant enterococci infection or a methicillin-resistant Staphylococcus aureus infection.
10. The method of claim 9 , wherein the antibiotic-resistant bacterial infection includes a multi-drug resistant vancomycin-resistant enterococci infection or a multi-drug resistant methicillin-resistant Staphylococcus aureus infection.
11. The method of claim 9 , wherein administering a primary dose of a microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection, administering a secondary dose of the microbiota restoration therapy composition to the patient with the antibiotic-resistant bacterial infection, or both includes administering the microbiota restoration therapy composition to the patient with an enema tube.
12. The method of claim 9 , wherein administering a primary dose of a microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection, administering a secondary dose of the microbiota restoration therapy composition to the patient with the antibiotic-resistant bacterial infection, or both includes orally administering the microbiota restoration therapy composition.
13. A method of treating an antibiotic-resistant bacterial infection, the method comprising:
manufacturing a microbiota restoration therapy composition;
wherein manufacturing a microbiota restoration therapy composition comprises mixing of an effective amount of fecal microbiota from a fresh human fecal sample from a human donor and polyethylene glycol at a concentration of 30-90 g/L;
administering one or more doses of the microbiota restoration therapy composition to a patient with an antibiotic-resistant bacterial infection.
14. The method of claim 13 , wherein the antibiotic-resistant bacterial infection includes a vancomycin-resistant enterococci infection.
15. The method of claim 13 , wherein the antibiotic-resistant bacterial infection includes a methicillin-resistant Staphylococcus aureus infection.
16. The method of claim 13 , wherein the antibiotic-resistant bacterial infection includes a multi-drug resistant bacterial infection.
17. The method of claim 13 , further comprising administering a second dose of the microbiota restoration therapy composition to the patient.