Methods for synthesizing nucleotide analogues with disulfide linkers

Inventors: Mong Sano Marma (Natick, MA); Jerzy Olejnik (Brookline, MA); Ilia Korboukh (Needham, MA)

Assignee: Qiagen Sciences, LLC

C07H19/10 C07C323/12 C07C323/60 C07D207/46 C07F7/1804 C07H19/14 C09B1/00 C09B5/2436 C09B11/24 C09B23/083 C09B23/166 C12Q1/6811 C12Q1/6869 C12Q1/6876 C07C2603/18 Y02P20/55

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Quick Facts

Patent No.: US 10,336,785
App. No.: 15/343,341
Granted: Jul 2, 2019
Kind: B2

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Abstract

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Claims (4)

1. A compound wherein the structure is:

2. A compound wherein the structure is:

3. A compound wherein the structure is:

4. A compound wherein the structure is:

Assignments (6)

RELEASE OF SECURITY INTEREST Recorded Mar 24, 2023

From: PERCEPTIVE CREDIT HOLDINGS III, LP

To: ISOPLEXIS CORPORATION

Reel/Frame 063235/0942 →

PATENT PURCHASE AGREEMENT Recorded Jul 30, 2021

From: QIAGEN SCIENCES, LLC