Waveguide-based optical scanning systems
A scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning senor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector.
1. A chip for detecting a biologically active analyte, comprising:
a substrate wherein the substrate comprises a plurality of excitation waveguides, and a plurality of collection waveguides, the excitation waveguides and collection waveguides crossing to form a two-dimensional array of intersection regions where an excitation waveguide and a collection waveguide cross and provide optical communication with the intersection region at each crossing;
an array of optical fibers connected to the collection waveguides to couple the collection waveguides to a detector; and
an array of optical fibers connected to the excitation waveguides to couple the excitation waveguides to a light source; and
a plurality of optical sensing sites each in optical communication with an intersection region.
2. The chip of claim 1 , wherein one of the optical sensing sites comprises a sensor and a reference sample material.
3. The chip of claim 1 , wherein a first light wave in an excitation waveguide is transduced by a sensor of an optical sensing site in optical communication with the excitation wave guide resulting in a second light wave in a collection waveguide.
4. The chip of claim 3 , wherein the sensor supports an immunoassay.
5. The chip of claim 4 , wherein the immunoassay supported on the sensor is a fluorescent immunoassay.
6. The chip of claim 3 , wherein the sensor is selected from the group consisting of a fluorescence well, an absorption cell, an interferometric sensor, a diffractive sensor and surface plasmon resonance sensor.
7. The chip of claim 1 , wherein the biologically active analyte is selected from the group consisting of a nucleic acid, a protein, an antigen, an antibody, a lipid, a polysaccharide, a glycoprotein, a cell, a tissue, a microorganism, a gas, a chemical agent and a pollutant.
8. The chip of claim 1 , wherein the crossing of the excitation waveguides and collection waveguides is substantially perpendicular.
9. The chip of claim 1 , wherein the excitation waveguides are single-mode and the collection waveguides are multi-mode.
10. The chip of claim 1 , wherein the excitation waveguides and the collection waveguides support single-mode in a first vertical dimension and multi-mode in a second lateral dimension.
11. The chip of claim 1 , wherein the excitation waveguides and the collection waveguides are multi-mode.
12. The chip of claim 1 , wherein the excitation waveguides and the collection waveguides are single-mode.
13. The chip of claim 1 , wherein the excitation waveguide comprises a plurality of branches for drawing a fraction of the light from a first light wave traveling in the excitation waveguide.
14. The chip of claim 1 , wherein the collection waveguide comprises a plurality of funnels for collecting light from the sensing sites and coupling it to the collection waveguide.
15. The chip of claim 1 , wherein the optical sensing sites comprise the surface of the substrate above the intersection region of the excitation waveguides and the collection waveguides.
16. The chip of claim 1 , wherein the optical sensing sites comprise biochemical interaction sites.
17. The chip of claim 1 , wherein the optical sensing sites comprise optical transducers.
18. The chip of claim 17 , wherein the optical transducers comprise wells comprising fluorescent compounds or luminescent compounds, wherein light waves guided by the excitation waveguides excite the fluorescent compounds or luminescent compounds in the wells in the presence of a biologically active analyte, and the collection waveguides collect and guide light emitted from the fluorescent compounds or the luminescent compounds in the wells to the detector.
19. The chip of claim 1 , wherein the number of optical sensing sites is greater than 10.
20. The chip of claim 1 , wherein the density of optical sensing sites is greater than 100 per cm 2 .
21. The chip of claim 1 , wherein the chip is in thermal communication with a thermal transfer element.
22. The chip of claim 21 , wherein the thermal transfer element is a thermoelectric cooler.
23. The chip of claim 1 , wherein each optical sensing site comprises a thermal transfer element in thermal communication with the optical sensing site.
24. The chip of claim 23 , wherein the thermal transfer element comprises a thin-film heater.
25. The chip of claim 23 , wherein each optical sensing site further comprises a thermistor in thermal communication with the optical sensing site.
26. The chip of claim 1 , wherein the substrate further comprises one or more microchannels and one or more reservoirs in fluid communication with one or more optical sensing sites.
27. The chip of claim 1 , wherein the chip further comprises a fluidics layer coupled to the substrate and comprises one or more microchannels and one or more reservoirs in fluid communication with one or more optical sensing sites.
28. The chip of claim 1 , wherein the optical sensing sites comprise a sensor, and wherein a measurable change in a first light wave results when the sensor discriminates or interacts with a sample comprising the biologically active analyte.