TREATMENT OF INTERVERTEBRAL DISC DEGENERATION USING HUMAN UMBILICAL CORD TISSUE-DERIVED CELLS
Methods for treating a patient having a disease or condition related to IVD degeneration are provided. The methods comprise administering cells obtained from human umbilical cord tissue, or administering pharmaceutical compositions comprising such cells or prepared from such cells. In some embodiments, administering the cells promotes repair and regeneration of degenerated IVD tissue in the patient. Pharmaceutical compositions for use in the inventive methods, as well as kits for practicing the methods are also provided.
1 . A method of treating a disease or condition related to intervertebral disc degeneration in a subject having a degenerated intervertebral disc comprising administering an isolated homogeneous population of cells obtained from human umbilical cord tissue into the degenerated intervertebral disc, wherein the umbilical cord tissue is substantially free of blood, wherein the isolated homogeneous population of cells is capable of self-renewal, and wherein the isolated homogeneous population of cells further has the following characteristics:
a. does not produce CD31, CD34, CD117, telomerase and HLA-DR;
b. expresses CD10, CD13, CD44, CD73, and CD90; and
c. expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, increased levels of interleukin 8 and reticulon 1.
2 . The method of claim 1 , wherein the isolated homogeneous population of cells expresses PDGFr-alpha and HLA-A,B,C.
3 . The method of claim 1 , wherein the cells are undifferentiated.
4 . The method of claim 1 , wherein the treating comprises promoting repair and regeneration of a degenerated intervertebral disc.
5 . The method of claim 1 , wherein the isolated homogeneous cell population is administered by injection.
6 . The method of claim 1 , wherein the isolated homogeneous cell population is administered with at least one other cell type and/or at least one agent.
7 . The method of claim 6 , wherein the at least one other cell type is administered simultaneously with, or before, or after, the isolated homogeneous cell population obtained from human umbilical cord tissue.
8 . The method of claim 6 , wherein the at least one agent is administered simultaneously with, before, or after administration of the isolated homogeneous cell population obtained from human umbilical cord tissue.
9 . The method of claim 6 , wherein the at least one agent is a trophic factor.
10 . The method of claim 9 , wherein the trophic factor is selected from the group consisting of: TGF-beta, GDF-5, PDGF-BB and TIMP1.
11 . The method of claim 9 , wherein the trophic factor exerts a trophic effect on the isolated homogeneous cell population obtained from human umbilical cord tissue.
12 . The method of claim 9 , wherein the trophic effect comprises increasing expression of one or more extracellular matrix proteins.
13 . The method of claim 1 , wherein the isolated homogeneous cell population is administered into the nucleus pulposus of the intervertebral disc.
14 . The method of claim 1 , wherein the isolated homogeneous cell population is administered into the annulus fibrosus of the intervertebral disc.
15 . The method of claim 1 , wherein the isolated homogeneous cell population has the ability to differentiate into cells displaying a nucleus pulposus cell phenotype.
16 . The method of claim 1 , wherein the isolated homogeneous cell population has the ability to differentiate into cells displaying an annulus fibrosus cell phenotype.