Aldehyde-tagged immunoglobulin polypeptides and methods of use thereof
The present disclosure provides aldehyde-tagged immunoglobulin (Ig) polypeptides that can be converted by a formylglycine-generating enzyme to produce a 2-formylglycine (FGly)-modified Ig polypeptide. An FGly-modified Ig polypeptide can be covalently and site-specifically bound to a moiety of interest to provide an Ig conjugate. The disclosure also encompasses methods of production of such aldehyde-tagged Ig polypeptides, FGly-modified Ig polypeptides, and Ig conjugates, as well as methods of use of same.
1. An immunoglobulin (Ig) polypeptide comprising an IgG1 heavy chain constant region comprising the amino acid sequence NSGALCTPSRGVHTFPAVLQSSGL (SEQ ID NO:235).
2. An antibody comprising the Ig polypeptide of claim 1 .
3. An Ig polypeptide comprising a 2-formylglycine (FGly) moiety, wherein the immunoglobulin (Ig) polypeptide comprises an IgG1 heavy chain constant region comprising the amino acid sequence NSGAL(FGly)TPSRGVHTFPAVLQSSGL (SEQ ID NO:239).
4. An antibody comprising the Ig polypeptide of claim 3 .
5. An antibody conjugate comprising an Ig conjugate, wherein the immunoglobulin (Ig) conjugate comprises an IgG1 heavy chain polypeptide and a covalently bound moiety,
wherein the IgG1 heavy chain polypeptide comprises a constant region comprising the amino acid sequence NSGAL(FGly′)TPSRGVHTFPAVLQSSGL (SEQ ID NO:244), and
wherein FGly′ is a formylglycine residue covalently bound to the moiety.
6. The antibody conjugate of claim 5 , wherein FGly′ has the formula:
wherein J1 is the covalently bound moiety;
each L1 is independently selected from alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, arylene, substituted arylene, cycloalkylene, substituted cycloalkylene, heteroarylene, substituted heteroarylene, heterocyclene, substituted heterocyclene, acyl, amido, acyloxy, urethanylene, thioester, sulfonyl, sulfonamide, sulfonyl ester, O, S, NH, and substituted amine; and
n is a number selected from zero to 40.
7. The antibody conjugate of claim 5 , wherein the moiety is selected from a drug, a detectable label, a water-soluble polymer, and a synthetic peptide.
8. The antibody conjugate of claim 5 , wherein the moiety is a small molecule drug.
9. The antibody conjugate of claim 8 , wherein the small molecule drug is a cancer chemotherapeutic agent.
10. The antibody conjugate of claim 5 , wherein the moiety is a water-soluble polymer.
11. The antibody conjugate of claim 10 , wherein the water-soluble polymer is poly(ethylene glycol).
12. The antibody conjugate of claim 5 , wherein the moiety is a detectable label.
13. The antibody conjugate of claim 12 , wherein the detectable label is an imaging agent.
14. The antibody conjugate of claim 5 , wherein the antibody specifically binds a tumor antigen on a cancer cell.
15. The antibody conjugate of claim 14 , wherein the moiety is a cytotoxic agent.
16. The antibody conjugate of claim 5 , wherein the antibody conjugate specifically binds an antigen on a cell infected by a virus.
17. The antibody conjugate of claim 16 , wherein the antigen is encoded by the virus.
18. The antibody conjugate of claim 5 , wherein the moiety is a viral fusion inhibitor.
19. A formulation comprising:
a) the antibody conjugate of claim 5 ; and
b) a pharmaceutically acceptable excipient.
20. The Ig polypeptide of claim 1 , comprising an IgG1 heavy chain constant region comprising the amino acid sequence set forth in SEQ ID NO: 259.