Device for generating droplets

1. A device for generating droplets, comprising:

a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface;

a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion;

a sample well, a carrier well, and a droplet well, wherein each well has an upper portion created by one of the protrusions; and

a cover layer attached to the bottom surface of the base portion and sealing a bottom side of each microfluidic channel;

wherein the plurality of microfluidic channels includes a first channel, a second channel, and a third channel that meet one another in a droplet-generation region, and

wherein the sample well is configured to supply sample-containing fluid to the droplet-generation region via the first channel, the carrier well is configured to supply carrier fluid to the droplet-generation region via the second channel, and the droplet well is configured to receive sample-containing droplets in carrier fluid from the droplet-generation region via the third channel.

2. The device of claim 1 , wherein the cover layer is thinner than the base portion.

3. The device of claim 1 , wherein the base portion and the plurality of protrusions are provided by an injection-molded polymer body.

4. The device of claim 3 , wherein each well defines a central axis and has a floor and a side wall region, wherein the side wall region surrounds the central axis and extends upwardly from the floor, and wherein the polymer body provides the side wall region of each well.

5. The device of claim 4 , wherein the polymer body provides the floor of each well.

6. The device of claim 4 , wherein the polymer body defines a plurality of openings each extending completely through the body in a direction orthogonal to the bottom surface of the base portion, and wherein each opening creates the side wall region of one of the wells.

7. The device of claim 6 , wherein each opening narrows at a position intermediate a top side of the body and the bottom surface of the base portion.

8. The device of claim 1 , where the plurality of microfluidic channels includes a fourth channel that meets the first channel, the second channel, and the third channel in the droplet-generation region.

9. The device of claim 8 , wherein the carrier well is configured to supply carrier fluid to the droplet-generation region via the second channel and the fourth channel.

10. The device of claim 1 , wherein the device comprises another well that is fluidically connected to the droplet well via the droplet-generation region.

11. A method of generating droplets, the method comprising:

selecting the device of claim 1 ; and

forming sample-containing droplets in the droplet-generation region.

12. A device for generating droplets, comprising:

an upper member injection molded as a single piece, the upper member defining a plane and a plurality of microfluidic channels arranged parallel to the plane;

a sample well, a carrier well, and a droplet well, each well defining a central axis and including a floor and a side wall region, the side wall region being formed by the upper member, the side wall region extending upwardly from the floor and surrounding the central axis; and

a lower member attached to the upper member and sealing a bottom side of each microfluidic channel;

wherein the plurality of microfluidic channels includes a first channel, a second channel, and a third channel that meet one another in a droplet-generation region, and wherein the sample well is configured to supply sample-containing fluid to the droplet-generation region via the first channel, the carrier well is configured to supply carrier fluid to the droplet-generation region via the second channel, and the droplet well is configured to receive sample-containing droplets in carrier fluid from the droplet-generation region via the third channel.

13. The device of claim 12 , wherein the upper member includes a substantially planar base portion having a bottom surface at which the lower member is attached to the upper member, and wherein the lower member is thinner than the base portion.

14. The device of claim 12 , wherein the upper member defines a plurality of openings each extending completely through the upper member, wherein the lower member covers a bottom end of each opening, and wherein each opening creates the side wall region of one of the wells.

15. The device of claim 14 , wherein each opening narrows at a position intermediate a top side and a bottom surface of the upper member.

16. The device of claim 12 , wherein the device comprises another well that is fluidically connected to the droplet well via the droplet-generation region.

17. The device of claim 12 , wherein the device includes a plurality of sample wells, a plurality of carrier wells, a plurality of droplet wells, and a plurality of droplet-generation regions, and wherein each droplet-generation region is connected to one of the sample wells, one of the carrier wells, and one of the droplet wells.

18. A method of generating droplets, the method comprising:

selecting the device of claim 12 ; and

forming sample-containing droplets in the droplet-generation region.

19. A device for generating droplets, comprising:

a body injection molded as a single piece, the body including a base portion and a plurality of annular protrusions projecting from a top surface of the base portion, the base portion defining a plurality of microfluidic channels;

a sample well, a carrier well, and a droplet well, wherein each well has an upper portion created by one of the annular protrusions; and

a cover layer attached to the base portion and sealing a bottom side of each microfluidic channel;

wherein the plurality of microfluidic channels includes a first channel, a second channel, and a third channel that meet one another in a droplet-generation region, and

wherein the sample well is configured to supply sample-containing fluid to the droplet-generation region via the first channel, the carrier well is configured to supply carrier fluid to the droplet-generation region via the second channel, and the droplet well is configured to receive sample-containing droplets in carrier fluid from the droplet-generation region via the third channel.

20. The device of claim 19 , wherein the cover layer is thinner than the base portion.

21. The device of claim 19 , wherein each well defines a central axis and has a floor and a side wall region, wherein the side wall region surrounds the central axis and extends upwardly from the floor, and wherein the body provides the side wall region of each well.

22. The device of claim 19 , where the plurality of microfluidic channels includes a fourth channel that meets the first channel, the second channel, and the third channel in the droplet-generation region.

23. The device of claim 19 , wherein the carrier well is configured to supply carrier fluid to the droplet-generation region via the second channel and the fourth channel.

24. The device of claim 19 , wherein the device includes a plurality of sample wells, a plurality of carrier wells, a plurality of droplet wells, and a plurality of droplet-generation regions, and wherein each droplet-generation region is connected to one of the sample wells, one of the carrier wells, and one of the droplet wells.

25. The device of claim 19 , wherein the device comprises another well that is fluidically connected to the droplet well via the droplet-generation region.

26. A method of generating droplets, the method comprising:

selecting the device of claim 19 ; and

forming sample-containing droplets in the droplet-generation region.