Systems and methods for interference correction from hemoglobin variants
A system for determining a concentration of hemoglobin A1C includes a first lateral flow test strip, the first lateral flow test strip providing for a percent of HbA1C concentration; a second lateral flow test strip, the second lateral flow test strip providing for the total amount of hemoglobin; an antibody-microparticle stripe on each of the first and second lateral flow test strips; a conjugate stripe on each of the first and second lateral flow test strips; and a sample treatment buffer. The sample treatment buffer is strongly denaturing, and antibodies in the antibody-microparticle strip are covalently bound to microparticles.
1. A system for determining a concentration of hemoglobin A1C, the system comprising:
a first lateral flow test strip, the first lateral flow test strip providing for a percent of HbA1C concentration and providing for the total amount of hemoglobin, an antibody-microparticle stripe on the first lateral flow test strip;
a conjugate stripe on the first lateral flow test strip; and
a sample treatment buffer, wherein the sample treatment buffer is denaturing, antibodies in the antibody-microparticle strip are covalently bound to microparticles, the microparticles include an aldehyde group, and the sample treatment buffer includes sodium perchlorate as an oxidizing agent.
2. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1M LiSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether, and 500 mM NaClO 4 .
3. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1M LiSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether and lithium perchlorate.
4. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1M LiSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether and sodium nitrite.
5. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1M LiSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether, and 2.0925 g/L K 3 Fe(CN) 6 .
6. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1.5M NaSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether, and 2.0925 g/L K 3 Fe(CN) 6 .
7. The system of claim 1 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 0.75M LiSCN, 0.75M NaSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether, and 2.0925 g/L KFeCN.
8. The system of claim 1 , further comprising:
a second lateral flow test strip, the second lateral flow test strip providing for a percent of HbA1C concentration and providing for the total amount of hemoglobin, an antibody-microparticle stripe on the second lateral flow test strip.
9. A system for determining a concentration of hemoglobin A1C, the system comprising:
a first lateral flow test strip, the first lateral flow test strip providing for a percent of HbA1C concentration and providing for the total amount of hemoglobin;
an antibody-microparticle stripe on the first lateral flow test strip;
a conjugate stripe on the first lateral flow test strip; and
a sample treatment buffer, wherein the sample treatment buffer is denaturing, and antibodies in the antibody-microparticle stripe are covalently bound to microparticles, and wherein the sample treatment buffer includes sodium perchlorate as an oxidizing agent.
10. The system of claim 9 , wherein the microparticles include an aldehyde group.
11. The system of claim 9 , wherein the sample treatment buffer includes 50 mM Imidazole, pH 7.0, 1M LiSCN, 0.5M GuSCN, 1% Polyoxyethyleneglycol Dodecyl Ether, and 500 mM NaClO 4 .
12. The system of claim 9 , wherein the antibody is monoclonal.