Compositions produced using enteric pathogens and methods of use
The present invention provides compositions including polypeptides having the characteristics of polypeptides expressed by a reference microbe such E. coli or Salmonella . Examples of Salmonella strains that can be used include, for instance, S. enterica serovar Newport, S. enterica serovar Enteritidis, S. enterica serovar Typhimurium, and S. enterica serovar Dublin. Also provided are compositions including polypeptides having a particular molecular weight and a mass fingerprint that includes polypeptide fragments having a particular set of masses, or polypeptides having an amino acid sequence with at least about 95% identity with an amino acid sequence, wherein the polypeptide has seroreactive activity. The present invention also provides methods of making and methods of using such compositions.
1. A composition comprising:
a first isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 92 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the first isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 904.51±250 parts per million (ppm), 908.44 ppm, 1050.50±250 ppm, 1078.54±250 ppm, 1171.60±250 ppm, 1276.56±250 ppm, 1343.65±250 ppm, 1403.73±250 ppm, 1450.67±250 ppm, 1479.76±250 ppm, 1510.72±250 ppm, 1546.66±250 ppm, 1567.75±250 ppm, 1624.79±250 ppm, 1668.72±250 ppm, 1739.90±250 ppm, 1822.89±250 ppm, 1858.93±250 ppm, 2122.02±250 ppm, and 2140.01±250 ppm;
a second isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 80 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the second isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 628.39±300 ppm, 830.45±300 ppm, 1177.61±300 ppm, 1633.84±300 ppm, 1786.85±300 ppm, 1796.82±300 ppm, 1870.95±300 ppm, 1980.96±300 ppm, 2087.91±300 ppm, 2091.10±300 ppm, 2173.92±300 ppm, 2302.02±300 ppm, and 2843.31±300 ppm;
a third isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 77 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the third isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 1380.59±400 ppm, 1525.72±400 ppm, 1688.88±400 ppm, 1748.79±400 ppm, 1831.90±400 ppm, 1889.03±400 ppm, 1929.04±400 ppm, 1968.03±400 ppm, 2030.93±400 ppm, 2917.36±400 ppm, and 2959.54±400 ppm;
a fourth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 72 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fourth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 628.39±400 ppm, 807.40±400 ppm, 871.47±400 ppm, 888.44±400 ppm, 1738.77±400 ppm, 1762.81±400 ppm, 1872.93±400 ppm, 1999.02±400 ppm, 2103.95±400 ppm, 2141.02±400 ppm, 2207.09±400 ppm, 2415.18±400 ppm, and 2439.29±400 ppm;
a fifth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 66 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fifth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 614.38±500 ppm, 715.38±500 ppm, 770.48±500 ppm, 830.38±500 ppm, 941.46±500 ppm, 951.48±500 ppm, 1025.62±500 ppm, 1134.55±500 ppm, 1154.67±500 ppm, 1221.63±500 ppm, 1335.64±500 ppm, 1395.64±500 ppm, 1531.71±500 ppm, 1656.77±500 ppm, 1676.80±500 ppm, 1716.88±500 ppm, 1778.88±500 ppm, 1962.95±500 ppm, 1997.02±500 ppm, 2261.08±500 ppm, 2397.13±500 ppm, and 3304.56±500 ppm; and
an effective amount of a pharmaceutically acceptable adjuvant.
2. The composition of claim 1 further comprising:
a sixth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 50 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the sixth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 787.46±450 ppm, 801.43±450 ppm, 827.45±450 ppm, 913.53±450 ppm, 1179.55±450 ppm, 1344.62±450 ppm, 1736.92±450 ppm, 1828.90±450 ppm, 2033.96±450 ppm, 2183.09±450 ppm, 2184.09±450 ppm, and 2226.06±450 ppm;
a seventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 42 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the seventh isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 631.37±400 ppm, 708.38±400 ppm, 715.40±400 ppm, 759.38±400 ppm, 930.49±400 ppm, 1002.51±400 ppm, 1019.54±400 ppm, 2248.09±400 ppm, 2642.29±400 ppm, 2700.24±400 ppm, and 2814.50±400 ppm;
an eighth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 38 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eighth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 704.42±500 ppm, 841.48±500 ppm, 884.41±500 ppm, 1289.57±500 ppm, 1438.68±500 ppm, 2353.10±500 ppm, and 2990.49±500 ppm;
a ninth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 36 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the ninth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 718.44±450 ppm, 867.42±450 ppm, 1057.56±450 ppm, 1248.54±450 ppm, 1438.68±450 ppm, 1933.77±450 ppm, 2217.05±450 ppm, 2389.09±450 ppm, and 2834.36±450 ppm;
a tenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 35 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the tenth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 817.43±400 ppm, 871.51±400 ppm, 1054.47±400 ppm, 1279.64±400 ppm, 1377.76±400 ppm, 1422.67±400 ppm, 1639.81±400 ppm, 2231.16±400 ppm, 2599.35±400 ppm, and 3477.67±400 ppm;
an eleventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 30 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eleventh isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 706.34±300 ppm, 776.48±300 ppm, 929.52±300 ppm, 964.57±300 ppm, 1065.58±300 ppm, 1108.55±300 ppm, 1204.61±300 ppm, 1403.81±300 ppm, 1560.82±300 ppm, 1575.79±300 ppm, and 2376.17±300 ppm;
a twelfth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 19 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the twelfth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 914.85±1 Da, 941.43±1 Da, 950.52±1 Da, 1019.54±1 Da, 1484.73±1 Da, 1516.89±1 Da, 1603.83±1 Da, 1675.81±1 Da, 1667.85±1 Da, and 2413.39±1 Da; or
a thirteenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 16 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the thirteenth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having m/z values of 602.30±1 Da, 914.52±1 Da, 941.43±1 Da, 950.52±1 Da, 1019.54±1 Da, 1042.61±1 Da, 1147.63±1 Da, 1362.63±1 Da, 1484.73±1 Da, 1516.89±1 Da, 1603.83±1 Da, 1675.81±1 Da, 1767.87±1 Da, 1931.94±1 Da, and 2262.14±1 Da.
3. A method of inducing production in an animal of antibody that specifically binds an iron-regulated polypeptide isolated from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, the method comprising:
administering to the animal an effective amount of the composition of claim 1 .
4. The method of claim 3 wherein the animal is avian, bovine, caprine, ovine, porcine, bisontine, cervine, equine, a companion animal, or human.
5. The method of claim 4 wherein the method further comprises collecting the antibody from the animal.
6. The method of claim 5 wherein the method further comprises isolating the antibody.
7. A composition comprising:
a first isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 92 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the first isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:880 to SEQ ID NO:893, SEQ ID NO:895, and SEQ ID NO: 897 to SEQ ID NO:901;
a second isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 80 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the second isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:902 to SEQ ID NO:914;
a third isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 77 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the third isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:915 to SEQ ID NO:922 and SEQ ID NO:924 to SEQ ID NO:926;
a fourth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 72 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fourth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:927 to SEQ ID NO:939;
a fifth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 66 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fifth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:940 to SEQ ID NO:953 and SEQ ID NO:955 to SEQ ID NO:962; and
an effective amount of a pharmaceutically acceptable adjuvant.
8. The composition of claim 7 further comprising:
a sixth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 50 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the sixth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:963 to SEQ ID NO:971 and SEQ ID NO:974 to SEQ ID NO:976;
a seventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 42 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the seventh isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:978 to SEQ ID NO:988;
an eighth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 38 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eighth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:989 to SEQ ID NO:995;
a ninth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 36 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the ninth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:996 to SEQ ID NO:1004;
a tenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 35 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the tenth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:1005 and SEQ ID NO:1007 to SEQ ID NO:1015;
an eleventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 30 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eleventh isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:1016 to SEQ ID NO:1020, SEQ ID NO:1022, SEQ ID NO:1023, and SEQ ID NO:1025 to SEQ ID NO:1028;
a twelfth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 19 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the twelfth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:1029 to SEQ ID NO:1037 and SEQ ID NO:1039; or
a thirteenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 16 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the thirteenth isolated iron-regulated polypeptide, when digested with trypsin, produces polypeptide fragments having the amino acid sequences of SEQ ID NO:1040 to SEQ ID NO:1054.
9. A method of inducing production in an animal of antibody that specifically binds an iron regulated polypeptide isolated from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, the method comprising:
administering to the animal an effective amount of the composition of claim 7 .
10. The method of claim 9 wherein the animal is avian, bovine, caprine, ovine, porcine, bisontine, cervine, equine, a companion animal, or human.
11. The method of claim 10 wherein the method further comprises collecting the antibody from the animal.
12. The method of claim 11 wherein the method further comprises isolating the antibody.
13. A composition comprising:
a first isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 92 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the first isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:880, SEQ ID NO:881, SEQ ID NO:882, SEQ ID NO:883, SEQ ID NO:884, SEQ ID NO:885, SEQ ID NO:886, SEQ ID NO:887, SEQ ID NO:888, SEQ ID NO:889, SEQ ID NO:890, SEQ ID NO:891, SEQ ID NO:892, SEQ ID NO:893, SEQ ID NO:895, SEQ ID NO:897, SEQ ID NO:898, SEQ ID NO:899, SEQ ID NO:900, and SEQ ID NO:901;
a second isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 80 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the second isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:902, SEQ ID NO:903, SEQ ID NO:904, SEQ ID NO:905, SEQ ID NO:906, SEQ ID NO:907, SEQ ID NO:908, SEQ ID NO:909, SEQ ID NO:910, SEQ ID NO:911, SEQ ID NO:912, and SEQ ID NO:914;
a third isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 77 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the third isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:915, SEQ ID NO:916, SEQ ID NO:917, SEQ ID NO:918, SEQ ID NO:919, SEQ ID NO:920, SEQ ID NO:921, SEQ ID NO:922, SEQ ID NO:924, and SEQ ID NO:926;
a fourth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 72 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fourth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:927, SEQ ID NO:928, SEQ ID NO:929, SEQ ID NO:930, SEQ ID NO:931 or SEQ ID NO:938, SEQ ID NO:932, SEQ ID NO:933, SEQ ID NO:934, SEQ ID NO:935, SEQ ID NO:936, SEQ ID NO:937, and SEQ ID NO:939;
a fifth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 66 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fifth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:940, SEQ ID NO:941, SEQ ID NO:943, SEQ ID NO:944, SEQ ID NO:945, SEQ ID NO:946, SEQ ID NO:947, SEQ ID NO:948, SEQ ID NO:949, SEQ ID NO:950, SEQ ID NO:951, SEQ ID NO:952, SEQ ID NO:953, SEQ ID NO:955, SEQ ID NO:956, SEQ ID NO:957, SEQ ID NO:958, SEQ ID NO:959, SEQ ID NO:960, SEQ ID NO:961, and SEQ ID NO:962; and
an effective amount of a pharmaceutically acceptable adjuvant.
14. The composition of claim 13 further comprising:
a sixth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 50 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the sixth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:963, SEQ ID NO:964, SEQ ID NO:965, SEQ ID NO:966, SEQ ID NO:967, SEQ ID NO:968, SEQ ID NO:969, SEQ ID NO:970, SEQ ID NO:971, SEQ ID NO:974, SEQ ID NO:975, and SEQ ID NO:976;
a seventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 42 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the seventh isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:978, SEQ ID NO:979, SEQ ID NO:980, SEQ ID NO:981, SEQ ID NO:982, SEQ ID NO:984, SEQ ID NO:985, SEQ ID NO:986, SEQ ID NO:987, and SEQ ID NO:988;
an eighth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 38 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eighth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:989, SEQ ID NO:990, SEQ ID NO:991, SEQ ID NO:992, SEQ ID NO:993, SEQ ID NO:994, and SEQ ID NO:995;
a ninth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 36 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the ninth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:996, SEQ ID NO:997, SEQ ID NO:998, SEQ ID NO:999, SEQ ID NO:1000, SEQ ID NO:1001, SEQ ID NO:1002, and SEQ ID NO:1003;
a tenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 35 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the tenth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:1005, SEQ ID NO:1007, SEQ ID NO:1008, SEQ ID NO:1009, SEQ ID NO:1010, SEQ ID NO:1011, SEQ ID NO:1012, SEQ ID NO:1013, SEQ ID NO:1014, and SEQ ID NO:1015;
an eleventh isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 30 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the eleventh isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO:1016, SEQ ID NO:1017, SEQ ID NO:1018, SEQ ID NO:1019, SEQ ID NO:1020, SEQ ID NO:1022, SEQ ID NO:1023, SEQ ID NO:1025, SEQ ID NO:1027, and SEQ ID NO:1028;
a twelfth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 19 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the twelfth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO: 1029, SEQ ID NO:1030, SEQ ID NO:1031, SEQ ID NO:1032, SEQ ID NO:1033, SEQ ID NO:1034, SEQ ID NO:1036, and SEQ ID NO:1037; or
a thirteenth isolated iron-regulated polypeptide from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, having a molecular weight of 16 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the thirteenth isolated iron-regulated polypeptide comprises the amino acid sequences of SEQ ID NO: 1040, SEQ ID NO:1041, SEQ ID NO:1042, SEQ ID NO:1043, SEQ ID NO:1044, SEQ ID NO:1047, SEQ ID NO:1048, SEQ ID NO: 1049, SEQ ID NO:1051, and SEQ ID NO:1052.
15. A method of inducing production in an animal of antibody that specifically binds an iron regulated polypeptide isolated from E. coli strain MS040330, deposited under ATCC accession number PTA-124620, the method comprising:
administering to the animal an effective amount of the composition of claim 13 .
16. The method of claim 15 wherein the animal is avian, bovine, caprine, ovine, porcine, bisontine, cervine, equine, a companion animal, or human.
17. The method of claim 16 wherein the method further comprises collecting the antibody from the animal.
18. The method of claim 17 wherein the method further comprises isolating the antibody.