Antibodies to risperidone and use thereof
Disclosed is an antibody or a binding fragment thereof that binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody or fragment thereof can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, quetiapine, olanzapine, and risperidone in a single lateral flow assay device.
1. An isolated antibody or a binding fragment thereof, which binds to risperidone, comprising:
a) the isolated antibody or the binding fragment thereof comprising a light chain variable region having the amino acid sequence of SEQ ID NO:3 and a heavy chain variable region having the amino acid sequence of SEQ ID NO:4; or
b) the isolated antibody or the binding fragment thereof comprising a light chain variable region having the amino acid sequence of SEQ ID NO:7 and a heavy chain variable region having the amino acid sequence of SEQ ID NO:8.
2. The isolated antibody or the binding fragment thereof of claim 1 , wherein the antibody or the binding fragment thereof comprises the light chain variable region having the amino acid sequence SEQ ID NO:3 and the heavy chain variable region having the amino acid sequence SEQ ID NO:4.
3. The isolated antibody or the binding fragment thereof of claim 1 , wherein the antibody or the binding fragment thereof comprises the light chain variable region having the amino acid sequence SEQ ID NO:7 and the heavy chain variable region having the amino acid sequence SEQ ID NO:8.
4. The isolated antibody or the binding fragment thereof of claim 1 , wherein the antibody or the binding fragment thereof comprises:
a) a light chain complementarity determining region (CDR) 1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:3,
b) a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:3,
c) a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:3,
d) a heavy chain CDR1 sequence comprising amino acid residues 50 to 54 of SEQ ID NO:4,
e) a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:4, and
f) a heavy chain CDR3 sequence comprising amino acid residues 118 to 128 of SEQ ID NO:4.
5. The isolated antibody or the binding fragment thereof of claim 1 , wherein the antibody or the binding fragment thereof comprises:
a) a light chain complementarity determining region (CDR) 1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:7,
b) a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:7,
c) a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:7,
d) a heavy chain CDR1 sequence comprising amino acid residues 50 to 54 of SEQ ID NO:8,
e) a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:8, and
f) a heavy chain CDR3 sequence comprising amino acid residues 118 to 128 of SEQ ID NO:8.
6. The isolated antibody or the binding fragment thereof of claim 1 , wherein the binding fragment is selected from the group of fragments consisting of Fv, F(ab′), F(ab′)2, scFv, minibody, and diabody fragments.
7. The isolated antibody or the binding fragment thereof of claim 1 , wherein the antibody is a monoclonal antibody.
8. An assay kit comprising the isolated antibody or the binding fragment thereof of claim 1 .
9. An assay device comprising the isolated antibody or the binding fragment thereof of claim 1 , wherein the isolated antibody or binding fragment thereof is disposed on the lateral flow assay device.
10. The assay device of claim 9 , wherein the assay device is a porous lateral flow assay device.
11. The assay device of claim 9 , wherein the assay device is a non-porous lateral flow assay device.
12. A method of detecting risperidone in a sample, the method comprising:
(i) contacting the sample with the isolated antibody or the binding fragment thereof of claim 1 labeled with a detectable marker, wherein the labeled antibody or the binding fragment thereof and risperidone present in the sample form a labeled complex; and
(ii) detecting the labeled complex thereby detecting risperidone in the sample.
13. The method of claim 12 , further comprising detecting the presence of one or more analytes in addition to risperidone.
14. The method of claim 13 , wherein the one or more analytes are anti-psychotic drugs other than risperidone.
15. The method of claim 14 , wherein the anti-psychotic drugs other than risperidone are selected from the group consisting of: paliperidone, quetiapine, olanzapine, aripiprazole, and metabolites thereof.
16. A competitive immunoassay method for detecting risperidone in a sample, the method comprising:
(i) contacting the sample with the isolated antibody or the binding fragment thereof of claim 1 , and with risperidone or a competitive binding partner of risperidone, wherein one of the antibody or binding fragment thereof and the risperidone or competitive binding partner thereof is labeled with a detectable marker, and wherein sample risperidone competes with the risperidone or competitive binding partner thereof for binding to the antibody or the binding fragment thereof to form a complex; and
(ii) detecting the amount of the detectable marker bound to the complex; and
(iii) determining the amount of sample risperidone based on the amount of the detectable marker.
17. The method of claim 16 , wherein the risperidone or competitive binding partner thereof is labeled with the detectable marker.
18. The method of claim 16 , wherein the antibody or the binding fragment thereof is labeled with a detectable marker.
19. The method of claim 16 , wherein the immunoassay is performed on a lateral flow assay device and the sample is applied to the device.
20. The method of claim 16 , further comprising detecting the presence of one or more analytes in addition to risperidone.
21. The method of claim 20 , wherein the one or more analytes are anti-psychotic drugs other than risperidone.
22. The method of claim 21 , wherein the anti-psychotic drugs other than risperidone are selected from the group consisting of: paliperidone, quetiapine, olanzapine, aripiprazole, and metabolites thereof.