Genes and gene signatures for diagnosis and treatment of melanoma
Panels of biomarkers, methods and systems are disclosed for determining gene expression, and diagnosing and treating melanoma.
1. A method for treating a patient having melanoma, the method comprising:
a) measuring in a tissue sample of the patient, expression of a panel of genes comprising a three gene combination PRAME, an S100-related gene, and an immune gene;
b) calculating a test score having a value greater than zero using a formula: score=1.22×PRAME+1.02×S100-related gene+0.7×immune gene+0.27, wherein gene expression values are normalized to housekeeper genes; and
(c) administering:
i. treatment to remove the melanoma site and at least a border of normal skin based on the test score being greater than or equal to 2;
ii. treatment to remove only the melanoma biopsy site based on the test score being less than 2 and greater than zero.
2. The method of claim 1 , wherein the immune gene is CXCL9.
3. The method of claim 1 , wherein the s100 related gene is S100A9.
4. The method of claim 1 , wherein measuring expression of a panel of genes comprises one or more of: microarray analysis, quantitative PCR and immunoanalysis.
5. The method of claim 1 , wherein the test score is generated using a linear model.