Genes encoding insecticidal proteins
The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a CryIC, CryIB or CryID protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other such chimeric genes.
1. A chimeric gene comprising the following operably-linked sequences:
(a) a coding region encoding a Cry1C protein comprising a DNA sequence with at least 98% sequence identity to the DNA of any one of SEQ ID Nos. 1, 3, 4 or 6, wherein said Cry1C protein is a protein comprising the amino acid sequence from the amino acid at position 29 to the amino acid at position 627 in SEQ ID No. 2, and
(b) a promoter region capable of directing expression in plant cells.
2. The chimeric gene of claim 1 , wherein said promoter comprises the sequence of SEQ ID No. 18 or 19.
3. The chimeric gene of claim 1 , further comprising a 3′ polyadenylation and transcript termination region.
4. A DNA comprising the chimeric gene of claim 1 , further comprising a second chimeric gene comprising the following operably-linked sequences:
(a) a second coding region encoding a Cry1B protein comprising a DNA sequence with at least 98% sequence identity to the DNA of SEQ ID No. 8 or 10, wherein said Cry1B protein is a protein comprising the amino acid sequence from the amino acid at position 31 to the amino acid at position 648 in SEQ ID No. 11, and
(b) a second promoter region capable of directing expression in plant cells.
5. A DNA comprising the chimeric gene of claim 1 , further comprising a second chimeric gene comprising the following operably-linked sequences:
(a) a coding region encoding a Cry1D protein comprising a DNA sequence with at least 98% sequence identity to the DNA of SEQ ID No. 12 or 14,
wherein said Cry1D protein is a protein comprising the amino acid sequence from the amino acid at position 21 to the amino acid at position 604 in SEQ ID No. 15, and
(b) a promoter region capable of directing expression in plant cells.
6. The DNA of claim 4 , wherein said second promoter region comprises the sequence of SEQ ID No. 18 or 19 and is different from said first promoter region.
7. A transgenic plant or seed, comprising the gene of claim 1 stably incorporated in its genome.
8. The plant or seed of claim 7 , which is a Brassica species plant or plant cell.
9. The plant or seed of claim 8 , wherein said plant is of the species Brassica oleraceae, Brassica napus, Brassica raga, Brassica juncea or Brassica carinata.
10. A method for controlling insects, comprising: planting or sowing in a field, plants comprising the chimeric gene of claim 1 .
11. A transgenic plant or seed comprising the DNA of claim 4 stably incorporated in its genome.
12. The plant or seed of claim 11 , wherein said plant is of the species Brassica oleraceae, Brassica napus, Brassica rape, Brassica juncea , or Brassica carinata.