Systems and methods of fluidic sample processing
The present invention provides fluidic devices and systems that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
1. A system, comprising:
a sample processing device, comprising:
at least a first reagent reservoir and a second reagent reservoir;
a manifold connecting to the first and second reagent reservoirs; and
a calibration unit;
a cartridge comprising an assay assembly having a reaction site; and
a removable sample collection unit,
wherein the manifold fluidically routes a first reagent and a second reagent from the first reagent reservoir and the second reagent reservoir, respectively, to the cartridge, and
wherein the calibration unit comprises electrodes and is configured to provide a conductivity calibration measurement of a sample such that the calibration measurement is configured to calibrate a detectable signal from the assay assembly.
2. The system of claim 1 , wherein the sample collection unit comprises a sample dilution syringe, the sample dilution syringe comprising a housing.
3. The system of claim 2 , wherein the sample dilution syringe contains a capillary tube, and wherein the capillary tube can be press-fit into the housing of the sample dilution syringe.
4. The system of claim 2 , wherein the sample dilution syringe further contains a moveable mixing element.
5. The system of claim 4 , wherein the moveable mixing element is a mixing ball.
6. The system of claim 1 , wherein the sample collection unit is fluidically connected to the reaction site of the assay assembly.
7. The system of claim 1 , wherein the sample collection unit further comprises a filter.
8. The system of claim 1 , wherein the device further comprises a body, and wherein the body covers the first reagent reservoir, second reagent reservoir, manifold, and cartridge.
9. The system of claim 1 , wherein first lower septum of the first reagent reservoir is held in place by a crimpable metal cap.
10. The system of claim 1 , wherein the first reagent reservoir and second reagent reservoir each contain 100-500 microliters of liquid reagent.
11. The system of claim 1 , wherein the first reagent reservoir comprises collapsible plastic.
12. The system of claim 1 , wherein the reaction site of the assay assembly comprises antibodies.
13. The system of claim 1 , wherein the device further comprises a waste chamber in fluidic communication with the assay assembly.
14. The system of claim 1 , wherein the cartridge comprises an opaque material.
15. The system of claim 1 , wherein the cartridge comprises a clear material.
16. The system of claim 1 , wherein the sample collection unit comprises a diluent.
17. The system of claim 1 , further comprising:
a detector configured to detect an optical signal generated via an interaction between the sample and at least one of the first and second reagents.
18. The system of claim 1 , wherein the sample is a bodily fluid, and wherein the calibration measurement provided by the calibration unit comprises a dilution of the sample.